Evaluation of Krill Meal in Commercial Diets for Juvenile Swimming Crab (Portunus trituberculatus)

Table 4

Real-time quantitative PCR primer-related genes of swimming crab (Portunus trituberculatus).

Genes names	Primers		Size (bp)	Access no.
	Forward (5-3)	Reverse (5-3)
β-actin	GAAGTAGCCGCCCTGGTTGT	GAATACCTCGCTTGCTCTGC	184	[25]
cat^a	GCTCTGACCCTGACTATGCT	CGACCAACAGGAATTAGCGG	180	FJ152102.2
gpx^b	TACCGTTCAGCAAGTACCGT	GTGGTGTTTTCCTGGTGACC	171	KY216076.1
cMnsod^c	GATCCATCACACCAAGCACC	GTTCTCCTCCAGCTTCAGGT	193	FJ031018.1
prx^d	TGACCAGCAACATGAGCAAC	CAATCTTGCGGAACTCCTCG	215	FJ174664.1
trx^e	GAAAGGGCGAGAAGGTCCA	CTACTCCCCAATGAGCCGAT	188	OP081649
tor^f	TGTGGACATAGGGCAAACTG	GACCGCTTCACCAAATCATC	174	[25]
akt^g	GGACTACGAGGCACCAAGAA	TGGACCACTTCATCACGCTC	179	[25]
s6k1^h	CGCCCCTCAGATTTCCAGT	TCTCAGCCTTTGTGTGCG	175	[25]
s6^i	CACTAACAACCGTGTGCGAC	ACCCTTCTTGATGATGACCAG	148	[25]
4e-bp1^j	GGCTGAACTTCCAAATGACT	TTCTTGGGTGGGGTCTTG	142	[25]
eif4e1a^k	TGAACAAGCAGCAGCGAG	TGACCACAGCACCACACA	114	[25]
eif4e2	GCTTTCAGGAGGACATCATC	TGAGGGAGTCATTGTGAGTCT	144	[25]
eif4e3	CCCTGGACCTTCTGGATTGA	TCCTGTTACCCCTCATCAAG	181	[25]

^acat: catalase; ^bgpx: glutathione peroxidase; ^ccMnsod: cytosolic manganese superoxide dismutase; ^dprx, thioredoxin peroxidase; ^etrx: thioredoxin; ^ftor: target of rapamycin; ^gakt: protein kinases B; ^hs6k1: ribosomal protein S6 kinase1; ⁱs6: ribosomal protein S6; ^j4e-bp1: eukaryotic initiation factor 4E binding protein-1; ^keif4e: eukaryotic translation initiation factor 4E.