Effects of Dietary Phospholipid and Cholesterol Levels on Growth, Molting Performance, and Ovary Development in Female Juvenile Crayfish (Procambarus clarkii)

Table 1

Feed formulations and approximate compositions of the experiment diets (g/kg dry matter).

Ingredients	Diets (phospholipid-cholesterol level (g/kg))
	Diet1	Diet2	Diet3	Diet4	Diet5	Diet6	Diet7	Diet8	Diet9
	(0/0)	(20/0)	(60/0)	(0/5)	(20/5)	(60/5)	(0/10)	(20/10)	(60/10)
Casein	300.0	300.0	300.0	300.0	300.0	300.0	300.0	300.0	300.0
Gelatin	75.0	75.0	75.0	75.0	75.0	75.0	75.0	75.0	75.0
Corn starch	385.0	385.0	385.0	385.0	385.0	385.0	385.0	385.0	385.0
Soybean oil	70.0	50.0	10.0	65.0	45.0	5.0	60.0	40.0	0.0
Soybean lecithin	0.0	20.0	60.0	0.0	20.0	60.0	0.0	20.0	60.0
Cholesterol	0.0	0.0	0.0	5.0	5.0	5.0	10.0	10.0	10.0
Cellulose	40.0	40.0	40.0	40.0	40.0	40.0	40.0	40.0	40.0
Choline chloride	5.0	5.0	5.0	5.0	5.0	5.0	5.0	5.0	5.0
Betaine	5.0	5.0	5.0	5.0	5.0	5.0	5.0	5.0	5.0
Vitamin premix^a	30.0	30.0	30.0	30.0	30.0	30.0	30.0	30.0	30.0
Mineral premix^b	20.0	20.0	20.0	20.0	20.0	20.0	20.0	20.0	20.0
CMC-Na	30.0	30.0	30.0	30.0	30.0	30.0	30.0	30.0	30.0
Yeast extract	40.0	40.0	40.0	40.0	40.0	40.0	40.0	40.0	40.0
Proximate composition (dry matter (g/kg))
Crude protein	401.1	409.9	403.8	405.1	409.6	400.1	403.4	409.9	409.2
Crude lipid	72.1	71.1	71.9	70.9	72.1	71.7	71.7	71.6	72.0
Moisture	105.5	101.2	101.0	103.7	103.9	104.6	103.4	104.9	103.3
Ash	49.1	51.3	53.2	48.8	51.8	53.7	49.2	50.6	56.4

^aVitamin premix provided the following per kg of diets: VA 62500 IU, VD₃ 15000 IU, VE 1.05 g, VK₃ 35.4 mg, VB₁ 100 mg, VB₂ 150 mg, VB₆ 150 mg, VB₁₂ 0.2 mg, VC 700 mg, biotin 4 mg, D-calcium pantothenate 250 mg, folic acid 25 mg, and nicotinamide 300 mg. ^bMineral premix provided the following per kg of diets: FeSO₄·H₂O 200 mg, CuSO₄·5H₂O 96 mg, ZnSO₄·H₂O 360 mg, MnSO₄·H₂O 120 mg, MgSO₄·H₂O 240 mg, KH₂PO₄ 4.2 g, NaH₂PO₄ 0.5 g, KI 5.4 mg, CoCl₂·6H₂O 2.1 mg, and Na₂SeO₃ 3 mg.