Effects of Dietary Phospholipid and Cholesterol Levels on Growth, Molting Performance, and Ovary Development in Female Juvenile Crayfish (Procambarus clarkii)

Table 6

Effects of dietary phospholipid and cholesterol levels on development performance in juvenile female P. clarkii.

PL (g/kg)	CH (g/kg)	Hepatopancreas		Ovary
		HSI (%)	Lipid (g/kg)	GSI (%)	E2 (ng/gprot)
0	0		^B	^A	^A
0	5		^B	^A	^B
0	10	^B		^A	^A
20	0		^AB	^AB	^A
20	5		^B	^A	^A
20	10	^B		^A	^A
60	0	^b	^b,A	^AB	^A
60	5	^ab	^a,A	^AB	^A
60	10	^a,A	^b	^B	^A
Pooled SE		0.14	2.72	0.20	0.47
Means of main effect
0		6.32	509.7^B	4.10^A	16.40
2		6.39	461.0^B	4.50^A	15.66
6		5.89	372.3^A	5.18^B	15.38
	0	6.56^b	483.3	4.60	15.10^a
	0.5	5.87^a	433.3	4.49	16.73^b
	1	6.18^ab	426.4	4.69	15.61^ab
Two-way ANOVA: P-values
PL		0.095	≤0.001	0.001	0.317
CH		0.023	0.081	0.771	0.060
PL × CH		0.427	0.010	0.075	0.011

PL: phospholipid; CH: cholesterol; HSI: hepatosomatic index; GSI: gonadosomatic index; lipid: total content of the lipid; E2: estradiol. Data are presented as the (). Values in the same line with different superscripts are significantly different (). ^ABSignificant difference between phospholipid levels within the same cholesterol level (). ^abSignificant difference between cholesterol levels within the same phospholipid level ().