Effects of Glycyrrhizin (GL) Supplementation on Survival, Growth Performance, Expression of Feeding-Related Genes, Activities of Digestive Enzymes, Antioxidant Capacity, and Expression of Inflammatory Factors in Large Yellow Croaker (Larimichthys crocea) Larvae

Table 2

Primer sequences of RT-qPCR used in this study.

Gene	Forward (5-3)	Reverse (5-3)	Reference
Npy	AAAGAGGTCCAGTCCTGAGATT	GTGGCGGCTCATAGTGGTAA	XM019275410
Agrp	GAGAGACAAGTGAGAGTTACCG	CGAAGAAGAAAGACGCAG	XM027281261.1
Trh	CGACGATGGAGACTGGGATA	CCTCGGCATGACTCTTGTTA	XM010755325.3
Ghrelin	TGACCTTGTGGTGCAAGTCGAC	CCGATTTCAAAGGGGGCACT	KC899122
Cart	GCCAACTGCAACTCCTACCT	TGCTACAGTAAATAAGCGGAAC	XM010741284.3
Leptin	GATGTTCTGGATGGACCTGC	AGACACCACTGATGCGGACT	XM010729765
Pomc	AGCCTGATTATCTGCCTCCC	CTTGTACGTGCCGTCCTTCT	XM027291739.1
Lepr	CAGCCCATTCATCTCCATTA	CTTGCCTCCTCTTCGTCTTC	XM019270027.2
Cck	TGGCTCCTCACTGTCTCACA	TTGCCTCAACAGACCCTGAT	XM019255482.2
Ifn-γ	TCAGACCTCCGCACCATCA	GCAACCATTGTAACGCCACTTA	XM019258900
Tnf-a	ACACCTCTCAGCCACAGGAT	CCGTGTCCCACTCCATAGTT	NM001303385
Il-1β	CATAGGGATGGGGACAACGA	AGGGGACGGACACAAGGGTA	XM010736551
Il-6	CGACACACCCACTATTTACAAC	TCCCATTTTCTGAACTGCCTCT	XM010734753
Il-8	AATCTTCGTCGCCTCCATTGT	GAGGGATGATCTCCACCTTCG	XM010737667.3
Il-10	AGTCGGTTACTTTCTGTGGTG	TGTATGACGCAATATGGTCTG	XM010738826
β-Actin	GACCTGACAGACTACCTCATG	AGTTGAAGGTGGTCTCGTGGA	GU584189

npy: neuropeptide Y; agrp: agouti-related protein; trh: thyrotropin-releasing hormone; cart: cocaine and amphetamine-regulated transcript; pomc: proopiomelanocortin; lepr: leptin receptor; cck: cholecystokinin; ifn-γ: interferon γ, tnf-a: tumor necrosis factor a; il-1β: interleukin-1β; il-6: interleukin-6; il-8: interleukin-8; il-10: interleukin-10.