Effects of Adiponectin on Glucose Metabolism in the Hepatopancreas of Grass Carp (Ctenopharyngodon idella)

Figure 4

Preparation of recombinant adiponectin proteins. Recombinant expression vector containing histidine-tagged grass carp adiponectin A or adiponectin B was transformed into BL21 strain, respectively, and the empty plasmid was transformed as negative control. The expression of recombinant adiponectin A (a) and adiponectin B (b) was induced by IPTG for 0, 1, 2, 3, and 4 h and analyzed by SDS-PAGE. Recombinant adiponectin A (c) and adiponectin B (d) were purified with Ni⁺–NTA resin and verified by SDS-PAGE.

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