Alteration of Growth Performance, Antioxidant Capacity, Tissue Fatty Acid Profiles, and Lipid Metabolism of Mud Crab (Scylla paramamosain) Juvenile in Response to Different Dietary Arachidonic Acid Levels

Table 1

Ingredients and proximate composition of the experimental diets (g kg^-1 dry matter).

Ingredients	Dietary ARA levels (g kg^-1)
	0.40	2.50	4.60	8.90	12.50	15.70
Peru fish meal^a	330.0	330.0	330.0	330.0	330.0	330.0
Soybean protein concentrate^a	260.0	260.0	260.0	260.0	260.0	260.0
Yeast extract^a	30.0	30.0	30.0	30.0	30.0	30.0
Krill meal^a	30.0	30.0	30.0	30.0	30.0	30.0
Wheat flour^a	238.0	238.0	238.0	238.0	238.0	238.0
ARA-enriched oil (50% ARA)^b	0.0	5.0	10.0	20.0	30.0	40.0
Palmitic acid^b	40.0	35.0	30.0	20.0	10.0	0.0
Soybean lecithin^c	10.0	10.0	10.0	10.0	10.0	10.0
Cholesterol	2.0	2.0	2.0	2.0	2.0	2.0
Ca (H2PO4)2	5.0	5.0	5.0	5.0	5.0	5.0
Vitamin premix^d	10.0	10.0	10.0	10.0	10.0	10.0
Mineral premix^e	20.0	20.0	20.0	20.0	20.0	20.0
Choline chloride	3.0	3.0	3.0	3.0	3.0	3.0
Sodium alginate	20.0	20.0	20.0	20.0	20.0	20.0
BHT^f	2.0	2.0	2.0	2.0	2.0	2.0
Proximate composition (g kg^-1 dry matter)
Moisture	99.6	95.1	91.8	91.9	98.9	96.5
Crude protein	484.9	488.4	481.0	488.5	489.7	483.4
Crude lipid	78.1	77.9	76.7	78.3	76.0	75.0
Ash	102.5	101.8	102.4	102.5	100.9	100.4

^aPeru fish meal (g kg^-1 dry matter): crude protein 707.7, crude lipid 87.8; Soybean protein concentrate (g kg^-1 dry matter): crude protein 690.9, crude lipid 4.3; yeast extract (g kg^-1 dry matter): crude protein 452.7, crude lipid 7.8; krill meal (g kg^-1, dry matter): crude protein 543.3, crude lipid 211.3; and wheat flour (g kg^-1 dry matter): crude protein 161.2, crude lipid 14.1. These ingredients were purchased from Ningbo Tech-Bank Feed Co., Ltd. (Ningbo, China). ^bARA-enriched oil and palmitic acid were purchased from Changsha Kenan Biotechnology Co., Ltd. (Changsha, China).^cSoybean lecithin was purchased from Ningbo Tech-Bank Feed Co., Ltd. (Ningbo, China). ^dVitamin premix (IU or g kg^-1 premix): retinyl acetate 2,500,000 IU; cholecalciferol 500,000 IU; all-rac-a-tocopherol 25,000 IU; menadione 5.63 g; thiamine 11.25 g; riboflavin 9.5 g; ascorbic acid 95 g; pyridoxine hydrochloride 10 g; cyanocobalamin 0.02 g; folic acid 2 g; biotin 0.375 g; nicotinic acid 37.5 g; D-calcium pantothenate 21.5 g; inositol 80 g; and ethoxyquin (antioxidant) 0.5 g. All ingredients were diluted with corn starch to 1 kg. ^eMineral mixture (g kg^-1 premix): FeC₆H₅O₇ 4.57 g; ZnSO₄·7H₂O 9.43 g; MnSO₄·H₂O (99%) 4.14 g; CuSO₄·5H₂O (99%) 6.61 g; MgSO₄·7H₂O (99%) 238.97 g; KH₂PO₄ 233.2 g; NaH₂PO₄ 137.03 g; C₆H₁₀CaO₆·5H₂O (98%) 34.09 g; and CoCl₂·6H₂O (99%) 1.36 g. All ingredients were diluted with corn starch to 1 kg. ^fBHT was purchased from Aladdin Industrial Co., Ltd. (Shanghai, China).