Garlic Powder Supplementation Improves Growth, Nonspecific Immunity, Antioxidant Capacity, and Intestinal Flora of Chinese Mitten Crabs (Eriocheir sinensis)

Table 2

Primer sequence for real-time PCR assays.

Genes^1	GenBank id	Primer sequence, sense/antisense	Product size (bp)
β-Actin	KM244725.1	TGGGTATGGAATCCGTTGGC; AGACAGAACGTTGTTGGCGA	101
CAT	GU361618.1	AATGAGGAAGAGCGGCAGAG; TCGGGACAAACATGAAAGAAGT	110
GPx	FJ617305.1	ATGCCAGAGTTCACTAAGAGG; AAGGGTCACAGCCAGGTC	165
TLR1	JX295852	ACTGTCTTGCTCGTCGTCTT; CTTATTGGCATCTGCTTCCT	135
TLR2	KC011816	GCATACCAGGACGACGAACA; TGAGCGAGGAGAGCTTGTTG	130
Myd88	KC019316	TGGCGTGCCGACTTGATT; TGACCCACCAGACTTCCT	178
TuBe	KC011815	GGTGGGTTACTCCTGTGACG; ACCCGCATCATCCATTCCAG	123
Dif	KC900086	CCCAATGTTTGTAGCACGGC; CTGGAGAGGCTAGTGAGGGT	192
Relish	GQ871279.1	TGGGTGTTACGAGTGCACAA; TGGTCAGAGTAGGGAGAGGC	118
Crustins	GQ200832.1	GCTCTATGGCGGAGGATGTCA; CGGGCTTCAGACCCACTTTAC	116
ALF	HQ850572.1	ACGAGGAGCAAGGAAAGAAAG; TTGTGCCATAGACCAGAGACTT	188
Lysozyme	JN416111.1	CTGGGATGATGTGGAGAAGTGC; TTATTCGGTGTGTTATGAGGGGT	111
proPO	EF493829.1	CCATGTCATCATTGCAGCGG; TGTACTTGTGCCAGCGGTAG	119

¹CAT = catalase; GPx = glutathione peroxidase; TLR 1, 2 = Toll-like receptors 1, 2; Myd88 = myeloid differentiation factor 88; ALF = antilipopolysaccharide factor, proPO = prophenoloxidase.