Effects of Dietary Lipid Levels on the Lipid Deposition and Metabolism of Subadult Triploid Rainbow Trout (Oncorhynchus mykiss)

Table 1

Formulation and proximate compositions of the experimental diets (g kg^-1).

Ingredients	LL diet (148 g kg^-1)	ML diet (228 g kg^-1)	HL diet (294 g kg^-1)
Fish meal^1	600	600	600
Wheat meal^1	120	120	120
Corn starch^1	142.2	62.2	2.2
Fish oil	80	160	220
Soybean oil	30	30	30
Mineral premix^2	5	5	5
Vitamin premix^3	5	5	5
Ca (H2PO4)2	8	8	8
Choline chloride	3	3	3
Mold inhibitor	1	1	1
Antioxidants	0.5	0.5	0.5
Betaine	5	5	5
Astaxanthin^4	0.3	0.3	0.3
Total	1000	1000	1000
Proximate analysis ()
Moisture (g kg^-1 diet)	37	43	42
Crude protein (g kg^-1 diet, dry basis)	461	458	455
Crude lipid (g kg^-1 diet, dry basis)	148	228	294
Ash (g kg^-1 diet, dry basis)	111	109	109
Gross energy (kJ g^-1 diet)	21.1	22.9	24.2

¹Fish meal; crude protein, 695 g kg^-1; crude lipid, 80 g kg^-1. Wheat meal; crude protein, 150 g kg^-1; crude lipid, 12 g kg^-1. Corn starch; crude protein, 3 g kg^-1; crude lipid, 2 g kg^-1. ²Mineral premix included the following (mg kg^-1 diet): sodium, 1500; iron, 3000; copper, 90; zinc, 1500; manganese, 800; selenium, 4.3; iodine, 21; and cobalt, 3. ³Vitamin premix included the following (each kg^-1 diet): vitamin A, 50 KIU; vitamin D3, 20 KIU; vitamin E, 390 mg; vitamin K, 150 mg; vitamin B1, 120 mg; vitamin B2, 165 mg; vitamin B6, 130 mg; vitamin B12, 0.5 mg; biotin, 2.4 mg; folic acid, 75 mg; inositol, 1200 mg; niacin, 670 mg; and ascorbic acid, 2500 mg. ⁴Astaxanthin: 10% (CAROPHYLL®, DSM, Netherlands).