Research Article

Effects of Dietary Lipid Levels on the Lipid Deposition and Metabolism of Subadult Triploid Rainbow Trout (Oncorhynchus mykiss)

Table 2

Primer sequences for real-time quantitative PCR.

Gene symbolGene namePrimer sequence (5-3)GenBank accession no.

Reference gene
β-Actinβ-ActinF: TACAACGAGCTGAGGGTGGCR: GGCAGGGGTGTTGAAGGTCTAJ438158.1
Lipid uptake
LPLLipoprotein lipaseF: GGACGTTGGGGAGCTGCTTAR: ATTGAGTCTCCCCGGCCTTGNM_001124604.1
CD36Cluster of differentiation 36F: GTCGTGGCTCAAGTCTTCCAR: TCAAATACTCGGCTCGCCTCAY606034.1
FATPFatty acid transport proteinF: TGCTGGTGGGAAGGATCAACR: TCCCCTGACAGATATGCCGAXM_021558559.1
FABPFatty acid binding proteinF: ACGGCAAGGAGACCAAGTTTR: TACGCACTGCCACAATGTCTJN413683.1
FABP3Fatty acid binding protein 3F: ATGGCTGAGGCATTCGR: CATCTTACCACCGTCTATCNM_001124713.1
FABP10Fatty acid binding protein 10F: CTTCGTCATCACCTCCAAR: CCACTGTCAGCGTCTCAXM_021595306.1
Triacylglycerol synthesis and catabolism
ACCαAcetyl-CoA carboxylase αF: CCCATTCGCCTTTTCCTTACR: ACCCTGCGTGTCCCCATAXM_021623129.1
FASFatty acid synthaseF: TCTAGAGACGCCACCTTCGAR: TGCAGTTTCTCCTCAGCCAGXM_021581290.1
MEMalic enzymeF: CCATTGGAGGGGCGTTTACTR: GGGGTTACTCAGGGCGAAAAXM_021597955.1
G6PDHGlucose-6-phosphate dehydrogenaseF: ACCACTTGCTCCAGATGCTCR: TACTGCCCCAACACCACATCXM_021615620.1
DGAT2Diacylglycerol O-acyltransferase 2F: TGCCCAGTGAACCGTAACTCR: CCCTTGCGGTTATTCAGGGTXM_021623520.1
ATGLAdipose triglyceride lipaseF: GCTGCAGAGTGTTCTTTCGCR: GGGAGTGTTGCAATTCCAGCKM980090.1
HSL1Hormone-sensitive lipase subtype 1F: CGACCTACTGCGTGGACTACR: TGGCCCATATTCCGCAGTTTNM_001197209.1
CPT1Carnitine palmitoyl transferase 1F: TACAGCTGGCCCAATTCAGGR: TCGCAGTGTTCTTGTCCTCCAF327058.3
ACOAcetyl-CoA oxidaseF: TTGGGCCTCATCATTGCAGTR: ACTGGGTCTGGTGCTCAATGXM_021613038.1
ACCβAcetyl-CoA carboxylaseF: CAGCTTGGTAAACAGCCTGGR: TCCGCACATTCTTCCAGTCCXM_021620985.1
VLDL assembly and lipoprotein clearance
ApoBApolipoprotein BF: TCCAGCCATCAACCTCCCTAR: TCGCCATAGAGCTTGCCAAAXM_021585085.1
MTPMicrosomal triglyceride transfer proteinF: ACGTGACTGTGGACATGGACR: ATCTGCATGCACACCAGGAAXM_014195517.1
HLHormone-sensitive lipaseF: CGACCTACTGCGTGGACTACR: TGGCCCATATTCCGCAGTTTNM_001197209.1
LDLRLow-density lipoprotein receptorF: GTGACAGGCAGGGACATCAAR: TGGCTGTTTGAGGTTGTGGTXM_021558198.1
LRP-1Lipoprotein receptor-related protein 1 likeF: GCACCAGAGCCTGTATGTGTR: AGAGCAGGAAGGAGCCCATAXM_021565892.1
SRBIScavenger receptor class BIF: TGGAGCAGAGAGGACCCTACR: GTGACCACATCCGACTCGTTXM_021590607.1
Transcription factors
PPARαPeroxisome proliferator-activated receptor αF: CACTCCACCCTTCGTCATCCR: CCTCAGCCTCCTTCTCAAGCHM536190.1
PPARβPeroxisome proliferator-activated receptor βF: GGGGTACACGTGTTCTACCGR: GTACTTCAGCAGCGTCACCTHM536191.1
PPARγPeroxisome proliferator-activated receptor γF: GAGCTGGACATGAACGACCAR: TGTGCCGTCCTTGTTCATGANM_001197212.1
SREBP1Sterol regulatory element-binding protein 1F: TCCTCTCCCTCAATCCCCTGR: CGAGTCAGCTGCGTTGTCTKP342261.1
LXRLiver X receptorF: CCAGAGTCAGGACCCTCAGAR: CTCCTGGACCGACATGATGGFJ470291.1
Carbohydrate metabolism
GKGlucokinaseF: AGATCACTGTGGGCATCGACR: GATGTCACAGTGAGGCGTCAAF053331.2
PKPyruvate kinaseF: GTTCCCTGTCGAGTCTGTGGR: CAGACGACGAAGCTCCTCAAXM_021622264.1
G6PaseGlucose-6-phosphataseF: GCTGACCTGCATACCACCTTR: CAGCCACCCAGATGAGCTTTXM_021575943.1
FBPaseFructose 1,6-bisphosphataseF: CCACTGGATGGATCTTCCAACAR: CCCTCTCATTGGGCTCATCGAF333188.1
PEPCKPhosphoenol pyruvate carboxykinaseF: CGGTGTGTTTGTAGGAGCCTR: ACGTGGAAGATCTTGGGCAGNM_001124275.1