Starvation Affects the Muscular Morphology, Antioxidant Enzyme Activity, Expression of Lipid Metabolism-Related Genes, and Transcriptomic Profile of Javelin Goby (Synechogobius hasta)

Figure 3

Effects of starvation on the muscle histological structure of Synechogobius hasta. Representative cross sections of muscle tissues of S. hasta fed as normal (a) and subjected to 3 (b), 7 (c), and 14 (d) days of starvation. The sections were stained with hematoxylin and eosin. MF: muscle fiber; EN: endomysium; EP: epimysium; dotted double arrow: long diameter (maximum length of the skeletal muscle parallel to the long axis of the skeletal muscle); solid double arrow: short diameter (maximum width of the skeletal muscle perpendicular to the long diameter). Scale bar: 200 μm. Length of long (e) and short (f) diameter in the muscle of S. hasta subjected to different starvation periods. All values were represented as () and analyzed using the Kruskal-Wallis ANOVA with Dunn’s test. Different uppercase letters denote significant differences ().

(a)

(b)

(c)

(d)

(e)

(f)