Research Article

Starvation Affects the Muscular Morphology, Antioxidant Enzyme Activity, Expression of Lipid Metabolism-Related Genes, and Transcriptomic Profile of Javelin Goby (Synechogobius hasta)

Table 1

qRT-PCR primers.

PrimerForward primer (5-3)Reverse primer (5-3)

fasCATCATCACTGGAGGTCTTGGATACGAATGCCTGATCTGGAAGT
scd1GACAACCAGCCCAAATCCGAGCCCCATCAGAAAGAC
lplAGTCCGATCAACACGAAGCGGTGCCGTTCCCATTTAG
cpt1aCGCTCCTGCTCCAATGAGAGAGACCACATAGAGGCAGAAGA
fatp1CCACTGGGCTCAGAATCAAGCAAGTTCAGCTCCAAAGACAATA
fabp3TGGCTGAAGCATTTGTTGGTGGGTCATCGGCAGTGGTCTCGT
srebp1TGCTATGCGGAGGTTATTCATCGTTGCTCTGCGTCGTAGTG
ppar γTTCTTCCACAGTTGCCAGTCGTTCATCAGAGGAGCCATCA
ahnakATCGCCACTGAGGAGAATAGGAGACAGCAGACTTACT
compCCACTTGTAGGAGGTCTTGGAACCAGGCATCCAACTC
gkTCTGAGGACTGAACAATGCGCTGAAGAACACTGGAACT
ltbp1GCCTCGCACTGCCAAGATGGCACGAGCACCTGAAACT
mapk9TTACCACATTCACCACAGTCTCCTCCTCTTCTTCCATAC
mct1CTTCGCACTCCTCTTGGGCAGCGTCATCCTCTTCATCT
mhcTTGGTTTGAGGAGGATGAGCTTAGGAGGCTGAGGAA
prkaa2ATGTAGAAGACGCCTCCTGCTCCAGAAGTCATCTCC
rxrβTCCAATGCCGTCTCAGAACAGGTGTAGGTCAGGTCTT
sfrp1CGCACCTTCAACAGACTTGAACTCCTACGCTCTAATCC
slc6a6AGTGGACTTGACGCCTTTCCCGCAAGAACAAGACCCT
slc40a1TGAACTACCTGCTGGACCGAGACGGAGATGATGACG
β-ActinGTGCGTGACATCAAGGAGAAGCGAGGAAGGATGGCTGGAA
gapdhGCCTCCTGCACCACAAACTGGACCATCCACGGTCTTCT
18 sTTCGATGGTACTTTCTGTGCCTGCCTTCCTTGGATGTG

Note: the primers sequences for the validation of DEGs in this study were designed using the Primer Premier 5. The primer sequences of the other genes in this study were described in previous studies [5, 17, 23] or designed by Primer Premier 5 software. The abbreviations in Table 1 were as follows: fas: fatty acid synthase; scd1: stearoyl-CoA desaturase 1; lpl: lipoprotein lipase; cpt1a: carnitine palmitoyltransferase 1 alpha; fatp1: fatty acid transport protein 1; fabp3: fatty acid binding protein 3; srebp1: sterol regulatory element binding protein 1; ppar γ: peroxisome proliferator-activated receptor γ; ahnak: neuroblast differentiation-associated protein (AHNAK); comp: cartilage oligomeric matrix protein; gk: glycerol kinase-like isoform X1; ltbp1: latent-transforming growth factor beta-binding protein 1-like; mapk9: mitogen-activated protein kinase 9-like isoform X2; mct1: monocarboxylate transporter 1-like; mhc: myosin heavy chain, fast skeletal muscle-like; prkaa2: 5-AMP-activated protein kinase catalytic subunit alpha-2; rxrβ: retinoic acid receptor RXR-beta-A isoform X1; sfrp1: secreted frizzled-related protein 1; slc6a6: sodium- and chloride-dependent taurine transporter-like isoform X2; slc40a1: solute carrier family 40 member 1; β-actin: beta-actin; gapdh: glyceraldehyde 3-phosphate dehydrogenase.