Dietary Leucine Supplementation Improves Muscle Fiber Growth and Development by Activating AMPK/Sirt1 Pathway in Blunt Snout Bream (Megalobrama amblycephala)

Table 2

Nucleotide sequences of primers used to quantify gene expressions by real-time PCR.

Target gene	Forward (5-3)	Reverse (5-3)	Accession number
bcat2	CCAAAGCCAGACCCTTCAAC	GGAAGGGCTTGATCTGAGGT	[83]
bckd-e2	AGCCACCATGTGTCGTTTCT	AGTGTCGGGATACGGAGTCA	[83]
ampkα1	AGTTGGACGAGAAGGAG	AGGGCATACAAAATCAC	[84]
ampkα2	ACAGCCCTAAGGCACGATG	TGGGTCGGGTAGTGTTGAG	[84]
sirt1	TCGGTTCATTCAGCAGCACA	ATGATGATCTGCCACAGCGT	[84]
myod	TTTGGGCAGCCTCTGGTTC	TGCTTCACCACCCACGACA	[85]
myf5	CTGTTGCAGTCAACCATGCT	AGGAACGCCATCCAGTACA	[85]
myog	TGGACAGCATTACAGGAACA	TGTTATGGTCGGTGAAAGG	[85]
mrf4	GGCAGCCTCTGGTTCGGAT	GCGGAGAGGAGCACGTCCT	[85]
mstna	AAGACAACCGGAGACCAAC	ATAGCGTTTCGGAGCAATA	[85]
mstnb	GATTTTCGGACTGAAGGAGA	TTCTGGGGGATGACAGTAAG	[85]
camk	TGGTGCGGAGATGTGTGAAA	GAGTAGGCGACAGATTCGGG	[39]
can	ACCTGGATAACTCGGGCTCT	AAGCTCGCCGTTGGATATGT	[39]
ef1α	CTTCTCAGGCTGACTGTGC	CCGCTAGCATTACCCTCC	X77689.1

bcat2: branched-chain amino transferase 2; bckd-e2: branch-chain α-keto acid dehydrogenase E2; ampkα1: AMP-activated protein kinase α1; ampkα2: AMP-activated protein kinase α2; sirt1: sirtuin 1; myod, myoblast determination protein; myf5: myogenic factor 5; myog: myogenin; mrf4: myogenic regulatory factor 4; mstna: Myostatin a; mstnb: Myostatin b; camk: Calcium/calmodulin-dependent protein kinase; can: Calcineurin; ef1α, elongation factor 1 alpha.