The Adaptive Characteristics of Cholesterol and Bile Acid Metabolism in Nile Tilapia Fed a High-Fat Diet

Figure 2

Effects of HFD on liver lipid profile and total bile acids in the liver and intestinal content of Nile tilapia after 4-week and 8-week feeding trials. (a) Oil red O staining of liver, scale bars: 100 μm (); (b) liver malondialdehyde (MDA) content (); (c) liver superoxide dismutase (SOD) enzyme activity (); (d) liver lipid content (); (e, f) liver triglyceride (TG) content and free fatty acid (FFA) contents (); (g–i) liver total cholesterol (TC), free cholesterol (FC), and cholesterol ester (CE) contents (); (j–l) liver low-density lipoprotein cholesterol (LDL-C), high-density lipoprotein cholesterol (HDL-C), and very-low-density lipoprotein (VLDL) contents (); (m) liver total bile acid (TBA) content (); (n, o) TC and TBA contents in intestinal content (). Data are represented as ; asterisks indicate the differences between the C and HFD groups in the same feeding trial, , , ; hash symbols indicate the differences between 4-week and 8-week feeding trials of the same lipid level diet, ^#, ^##, ^###; C: control diet; HFD: high-fat diet.

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