Dietary Niacin Requirement of Juvenile Chinese Mitten Crab, Eriocheir sinensis

Table 1

Ingredient formulation and proximate composition of the six experimental diets fed to Eriocheir sinensis (% dry matter).

Ingredients	Content (%)
	G1	G2	G3	G4	G5	G6
Casein (vitamin-free)	45.00	45.00	45.00	45.00	45.00	45.00
Dextrin	23.00	23.00	23.00	23.00	23.00	23.00
Carboxymethyl cellulose	13.12	13.10	13.08	13.06	13.04	13.00
Choline chloride	0.50	0.50	0.50	0.50	0.50	0.50
Cholesterol	0.50	0.50	0.50	0.50	0.50	0.50
Lecithin	1.50	1.50	1.50	1.50	1.50	1.50
Dimethyl-beta-propiothetin	0.30	0.30	0.30	0.30	0.30	0.30
Amino acid premix^a	0.38	0.38	0.38	0.38	0.38	0.38
Mineral premix^b, niacin-free	3.00	3.00	3.00	3.00	3.00	3.00
Vitamin premix^c	2.00	2.00	2.00	2.00	2.00	2.00
Fish oil	4.00	4.00	4.00	4.00	4.00	4.00
Soybean oil	2.00	2.00	2.00	2.00	2.00	2.00
Guar gum	1.50	1.50	1.50	1.50	1.50	1.50
Calcium dihydrogen phosphate	2.00	2.00	2.00	2.00	2.00	2.00
L-Ascorbic acid-2-trisodium phosphate	0.50	0.50	0.50	0.50	0.50	0.50
D-α-Tocopherol acetate	0.10	0.10	0.10	0.10	0.10	0.10
Inositol	0.60	0.60	0.60	0.60	0.60	0.60
Niacin	0.00	0.2	0.4	0.6	0.8	1.2
Total	100	100	100	100	100	100
Proximate composition (% dry matter)
Moisture	8.78	8.87	8.66	8.67	8.40	8.62
Crude protein	42.38	44	44.19	42.53	42.53	42.78
Crude lipid	5.36	5.2	5.26	5.29	5.31	5.31
Ash content	6.83	6.77	6.45	6.7	6.69	6.51
Niacin (mg/kg diet)	0.89	170.54	347.05	587.59	784.85	1248.86

^aAmino acid premix (per 1 kg diet): arginine, 1.8 g; valine, 0.9 g. ^bMineral premix (per 1 kg diet): Ca (H₂PO₄)₂, 10 g; MgSO₄·7H₂O, 2.4 g; KCl, 4.5 g; NaCl, 2.1 g; FeSO₄·H₂O, 155 mg; CuSO₄·5H₂O, 40 mg; ZnSO₄·H₂O, 80 mg; Mn·SO₄·H₂O, 30 mg; KI, 11.7 mg; CoCl₂·6H₂O, 4.8 mg; Na₂SeO₃, 2.4 mg. ^cVitamin premix (per 1 kg diet): vitamin A, 10000 IU; vitamin D, 2500 IU; vitamin K, 64 mg; thiamine, 60 mg; riboflavin, 250 mg; pyridoxine, 60 mg; calcium pantothenate, 240 mg; folic acid, 12 mg; biotin, 50 mg; cyanocobalamin, 4 mg.