An Evaluation of Replacing Soybean Meal with Proteolytic Soybean Meal in Low-Fish-Meal Diet on Growth Performance, Expression of Immune-Related Genes, and Resistance against Vibrio alginolyticus in White Shrimp (Litopenaeus vannamei)

Table 1

Composition of the experimental diets (g/kg dry matter basis).

Ingredients	Treatments
	Control	35	45	55	65
Brown fish meal^a	50	50	50	50	50
Corn protein powder^a	80	80	80	80	80
Soybean meal^a	305	270	260	250	240
Peanut meal^a	80	80	80	80	80
Spray-dried blood powder^a	35	35	35	35	35
Beer yeast cell^a	40	40	40	40	40
Meat meal^a	40	40	40	40	40
Methionine^a	1	1	1	1	1
Flour^a	235.7	235.7	235.7	235.7	235.7
[Ca(H2PO4)2]^a	20	20	20	20	20
Soybean lecithin^a	20	20	20	20	20
Fish oil^a	35	35	35	35	35
Squid paste^a	30	30	30	30	30
Mineral premix^b	15	15	15	15	15
Vitamin premix^c	10	10	10	10	10
Choline chloride^a	3	3	3	3	3
VC phosphate^a	0.3	0.3	0.3	0.3	0.3
Proteolytic soybean meal^d	0	35	45	55	65
Total	1000	1000	1000	1000	1000
Proximate compositions (%)
Moisture	11.84	12.37	12.12	12.29	12.09
Ash	7.73	7.36	7.53	7.31	7.18
Crude protein	39.55	39.28	39.45	39.14	39.59
Total lipid	12.40	12.54	12.76	12.70	12.19

^aGuangdong Yuehai Feed Co., Ltd. ^bPer kg mineral premix contains: 0.8 g Co, 0.02 g Se, 3 g Cu, 10 g Zn, 3.8 g Mn, 1 g Fe, 12 g Mg, 90 g K, and 10.5 g Ca. ^cPer kg vitamin premix contains: 8 million IU of vitamin A, 5 g thiamine-HCl, 15 g riboflavin, 2 million IU of cholecalciferol, 50 g DL-α-tocopherol, 8 g pyridoxine-HCl, 10 g menadione, 0.02 g cyanocobalamin, 40 g nicotinamide, 25 g Ca-pantothenate, 2.5 g folic acid, 0.08 g biotin, and 100 g inositol. ^dTaizhou Fuyi Biological Co., Ltd.