Replacement of Dietary Fishmeal Protein with Degossypolized Cottonseed Protein on Growth Performance, Nonspecific Immune Response, Antioxidant Capacity, and Target of Rapamycin Pathway of Juvenile Large Yellow Croaker (Larimichthys crocea)

Table 1

Formulation and approximate composition of the experimental diets.

	Diets (g/kg dry matter)
Ingredients	FM	DCP20	DCP40	DCP60	DCP80
White fish meal^1	384.00	307.00	230.00	153.00	77.00
Degossypolized cottonseed protein^2	0.00	89.00	175.00	261.00	349.00
Krill meal^1	35.00	35.00	35.00	35.00	35.00
Casein	101.00	101.00	101.00	101.00	101.00
Bread flour^1	256.00	256.00	256.00	256.00	256.00
Soy lecithin	20.00	20.00	20.00	20.00	20.00
Fish oil	51.00	58.00	66.00	73.00	80.00
Choline chloride	2.00	2.00	2.00	2.00	2.00
α-Starch	111.00	92.00	75.00	59.00	40.00
Vitamin C	1.00	1.00	1.00	1.00	1.00
Vitamin premix^1	2.00	2.00	2.00	2.00	2.00
Mineral premix^1	10.00	10.00	10.00	10.00	10.00
Ca(H2PO4)2	20.00	20.00	20.00	20.00	20.00
Attractant^1	5.00	5.00	5.00	5.00	5.00
Mould inhibitor^3	1.00	1.00	1.00	1.00	1.00
Ethoxyquinoline	1.00	1.00	1.00	1.00	1.00
Total	1000.00	1000.00	1000.00	1000.00	1000.00
Proximate analysis (g/kg dry matter)
Crude protein	418.20	418.90	418.80	418.60	419.70
Crude lipid	120.30	120.10	120.80	120.60	120.40
Crude ash	131.63	130.50	132.75	135.63	131.42
Carbohydrate	165.69	165.69	165.69	165.69	165.69
Gross energy^4 (kJ/g)	17.49	17.49	17.52	17.52	17.52

¹The crude protein and crude lipid content and detailed compositions of ingredients were presented in the previous study of He et al. [45]. ²Degossypolized cottonseed protein (649.00 g/kg crude protein, 5.00 g/kg crude lipid, 0.06 g/kg free gossypol, 25.40 g/kg valine, 7.40 g/kg methionine, 18.30 g/kg isoleucine, 34.30 g/kg leucine, 18.70 g/kg threonine, 33.10 g/kg phenylalanine, 16.80 g/kg histidine, 24.70 g/kg lysine, and 72.2 g/kg arginine). The degossypolized cottonseed protein was obtained from Da Bei Nong Bio-Tech Co., Ltd., China. ³The mould inhibitor is calcium propionate. ⁴The value of gross energy was calculated based on the contents of crude protein, crude lipid, and carbohydrate in diets.