Replacement of Dietary Fishmeal Protein with Degossypolized Cottonseed Protein on Growth Performance, Nonspecific Immune Response, Antioxidant Capacity, and Target of Rapamycin Pathway of Juvenile Large Yellow Croaker (Larimichthys crocea)

Table 2

Primer sequences used for RT-qPCR in the present study¹.

Target genes	Forward primers (5-3)	Reverse primers (5-3)	Reference
il-1β	CATAGGGATGGGGACAACGA	AGGGGACGGACACAAGGGTA	[21]
il-6	CGACACACCCACTATTTACAAC	TCCCATTTTCTGAACTGCCTCT	[21]
il-8	AATCTTCGTCGCCTCCATTGT	GAGGGATGATCTCCACCTTCG	[21]
il-10	AGTCGGTTACTTTCTGTGGTG	TGTATGACGCAATATGGTCTG	[21]
tnf-α	ACACCTCTCAGCCACAGGAT	CCGTGTCCCACTCCATAGTT	[21]
ifn-γ	TCAGACCTCCGCACCATCA	GCAACCATTGTAACGCCACTTA	[21]
tor	GCTGCAGTGTTGGTGTTGAG	GGACCCTGTCGTCTCGATTC	XM_027288345.1
s6	AGAAGCGTATGGCCACTGAG	CAGGAGTGTCCCTTGCTGAG	XM_019267468.2
4e-bp1	TGACCATCAACGACTCGGC	CCTGGAATGTTGGGCAGACC	XM_010732553.3
β-Actin	GACCTGACAGACTACCTCATG	AGTTGAAGGTGGTCTCGTGGA	[21]

¹il-1β: interleukin-1β; il-6: interleukin-6; il-8: interleukin-8; il-10: interleukin-10; tnf-α: tumor necrosis factor-α; ifn-γ: interferon-γ; tor: target of rapamycin; s6: ribosomal protein S6; 4e-bp1: eukaryotic initiation factor 4E binding protein 1.