Research Article
Optimal Niacin Requirement of Oriental River Prawn Macrobrachium nipponense as Determined by Growth, Energy Sensing, and Glycolipid Metabolism
Table 2
Nucleotide sequences of the primers used to quantify gene expressions by real-time PCR.
| | Target genes | Primer sequence | Accession number or reference |
| | ampkα | Forward (5-3) | TCACAGGGACAAAGGTAGCG | Sun et al., [50] | | Reverse (5-3) | TCTGCGAGCTTCCGATTCTT |
| | sirt1 | Forward (5-3) | AGGCTCGAGAACCACTTTGG | MT505387.1 | | Reverse (5-3) | GAAGTCCGACAAGCCACTCA |
| | glut2 | Forward (5-3) | CTGACTGTGCCGTCGTACAT | CP062040.1 | | Reverse (5-3) | ACACATAGGCCCACAGGTTG |
| | hk | Forward (5-3) | CATGGACAGCAGGATCTAC | KT932419 | | Reverse (5-3) | AAACTGAACGTGAAGCCTAA |
| | pk | Forward (5-3) | TAAGGGACCTGAAATTCGTA | KP690140 | | Reverse (5-3) | CCATCATCAACAAAAATCCT |
| | gs | Forward (5-3) | TTGAACCTTCGCGACCATGA | KP690145.1 | | Reverse (5-3) | CTGGCCTGGAGTGGCAATAA |
| | pc | Forward (5-3) | CACCATTTGTAGCACACAAC | KP690141 | | Reverse (5-3) | AACTCCATTTGCACCTCTAA |
| | pepck | Forward (5-3) | GGCATGGCGTAATGGTAGGA | JX435469.1 | | Reverse (5-3) | ACCTTACCCTTGTGTTCCGC |
| | g6p | Forward (5-3) | CGTGGACCTTTCTTCATTAG | KP690144 | | Reverse (5-3) | ACCATCAACCATTTGAGAAG |
| | fas | Forward (5-3) | CGTTCATGCTCGCGCAAATA | MK307767.1 | | Reverse (5-3) | CGCCCACCTTAGTTCCAGTT |
| | acc | Forward (5-3) | CAAGGTCCACTACATGGTCT | KP690138 | | Reverse (5-3) | ACTCTTCCCAAACTCTCTCC |
| | cptI | Forward (5-3) | AATTTTTGACTGGCTTCTCC | KP690136 | | Reverse (5-3) | TCCATTCTGGAAATCATCTG |
| | hsl | Forward (5-3) | GAAGGCCAGCGCTAATTTCG | MK633965.1 | | Reverse (5-3) | TCGAACCACCCATGAGAAGC |
| | β-Actin | Forward (5-3) | GTGCCCATCTACGAGGGTTA | FL589653.1 | | Reverse (5-3) | CGTCAGGGAGCTCGTAAGAC |
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ampkα: AMP-activated protein kinase α; sirt1: sirtuin 1; glut2: glucose transporter 2; hk: hexokinase; pk: pyruvate kinase; gs: glycogen synthase; pc: pyruvate carboxylase; pepck: phosphoenolpyruvate carboxykinase; g6p: glucose-6-phosphatase; fas: fatty acid synthase; acc: acetyl-CoA carboxylase; cptI: carnitine palmitoyltransferase I; hsl: hormone-sensitive lipase.
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