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Aquaculture Nutrition
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Aquaculture Nutrition
/
2022
/
Article
/
Tab 2
/
Research Article
Effects of Dietary Sodium Acetate on Growth Performance, Fillet Quality, Plasma Biochemistry, and Immune Function of Juvenile Golden Pompano (
Trachinotus ovatus
)
Table 2
Primer sequences used for qRT-PCR analysis in this study.
Genes
Sequence
Length (bp)
TM (°C)
Source
TGF-
β
F
TATCCCTCTACAACAGCACCA
21
55.61
[
41
]
R
GGTCAGCAGGCGGTAATC
18
57.18
IL-8
F
GAGAAGCCTGGGAATGGA
18
54.90
[
41
]
R
GAGCCTCAGGGTCTAAGCA
19
57.32
TNF-
α
F
CGCAATCGTAAAGAGTCCCA
20
55.40
[
41
]
R
AAGTCACAGTCGGCGAAATG
20
55.40
Keap1
F
AGAGGATGGAGATGGCACAG
20
57.45
[
41
]
R
CATTGGTTGGTCTTGGGATT
20
53.35
Nrf2
F
AGCTTGGCCTTCATCAAAT
19
50.85
[
41
]
R
GAGTATGGCTGTCCTTCTTCA
21
55.61
NF-
κ
B
F
TGCGACAAAGTCCAGAAAGAT
21
53.66
[
41
]
R
CTGAGGGTGGTAGGTGAAGGG
21
61.47
β
-Actin
F
TACGAGCTGCCTGACGGACA
20
59.50
[
48
]
R
GGCTGTGATCTCCTTCTGC
19
57.32