Two Strains of Gibel Carp (Carassius gibelio) Exhibit Diverse Responses to Carbohydrates in a Low-Lipid Diet

Table 2

Primer sequences and accession numbers for qPCR analysis.

Gene name	Forward primer (5-3)	Reverse primer (5-3)	GenBank accession number
Reference gene
ef1α	GTTGGAGTCAACAAGATGGACTCCAC	CTTCCATCCCTTGAACCAGCCCAT	AB056104
Glucose transport
glut2	CTCGTGGATGAGCTACCTCAGCAT	CCCTGACTGAAGATCTCCGCCA	KX898504
Glycolysis
gck	GAGGAGATGCGTAAGGTGGAGCT	TTCTCATACAGCTGATGTCCAGGGTT	KX898498
pfk	ACACCGGATGCCGCAGAAGCA	TCGATCTCTCCGGTCACATACTCG	KX898500
pk	GCATCTGTGTCTGCTGGACATCGA	TGAGAGCCGTGAGAGAAGTTCAGTC	KX898502
Gluconeogenesis
pepck	AGACAAACCCTCATGCCATGGCAAC	GGGTCTATGATGGGGCACTGG	KX898506
fpb	CACAAATGTTACAGGTGACCAGGTGAA	AATGTTTGAAGAGCCATCCAGAGGGT	GU593002
g6p	CCTTACTGGTGGGTCCATGAGACT	TGGGCCGGTCTCACAGGTCAT	KX898505
Lipogenesis
srebp-1	GGCCCTCTACTGCGTGGCACA	ACCACCATTTGGAGTGAGGGTCAC	KX898507
acly	AGTTTGGCCACGCTGGAGCTTGT	CCCAGCTCATCGAAGCTCTTGG	KX898508
fas	CCACACCATGGACCCACAGCT	CTGGGTCTTTACTGAAGGCCTCT	KF511494
Fatty acid oxidation
cpt1a	GAAGCTCATCAGGCTGTGGCCTT	TTCCAGGAGTGAAGTCCGGAGAG	KX898509
aco3	TGTGGAGGACACGGTTACCTTGC	AGTTGCTGGTCTGCTGCAGAAGG	KX898510

Reference gene: ef1α: elongation factor-1 alpha. Glucose transporter: glut2: glucose transporter 2. Glycolysis: gck: glucokinase; pfk: 6-phosphofructokinase; pk: pyruvate kinase. Gluconeogenesis: pepck: phosphoenolpyruvate carboxykinase; fbp: fructose 1,6-bisphosphatase; g6p: glucose-6-phosphatase. Lipogenesis: srebp-1: sterol regulatory element binding protein 1; acly: ATP citrate lyase; fas: fatty acid synthase. Fatty acid oxidation: cpt1a: carnitine palmitoyl transferase 1 isoform a; aco3: acyl-CoA oxidase 3.