Study on Carbohydrate Metabolism in Adult Zebrafish (Danio rerio)

Figure 2

Excess carbohydrates elevated lipid accumulation in zebrafish and ZFL (means ± SEM, n = 6). C0, C10, C20, C30, C40, and C50 represent diets in which dextrin levels are 0%, 10%, 20%, 30%, 40%, and 50%, respectively. L represents a linear trend, Q represents a quadratic trend, C represents a cubic trend, and # represents the significant of the corresponding regressions. HG represents high glucose. (a) Plasma triglycerides, (b) plasma cholesterol, (c) representative images of the liver oil red O staining, scale bar, 50 μm, (d) relative areas of lipid droplets in oil red O staining, (e) triglycerides in the liver of zebrafish, (f) representative images of BODIPY staining in ZFL, scale bar, 5 μm, (g) lipid content was quantified by mean green fluorescence intensity by BODIPY 493/503 staining, and (h) triglycerides content in ZFL. Labeled means without a common letter differ among C0, C10, C20, C30, C40, and C50, (one-way ANOVA, Duncan’s post hoc test). (two-tailed independent t-test).

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