Research Article
Study on Carbohydrate Metabolism in Adult Zebrafish (Danio rerio)
Table 2
Sequences of the primers used for qRT-PCR.
| | Gene name | Forward (F) and reverse (R) primer (5′–3′) | Accession no. | Product length (bp) |
| | Ribosomal protein L7 (rpl7) | F: CAGAGGTATCAATGGTGTCAGCCC | NM_213644.2 | 119 | | R: TTCGGAGCATGTTGATGGAGGC |
| | Hexokinase 1 (hk1) | F: CTTGGGTGTAGAGCCGTCTG | NM_213252.1 | 142 | | R: ACGTGGGGTGTTCTTGTTGT |
| | Glucokinase (gck) | F: CACCGCTGACCTGCTATGAT | NM_001045385.2 | 102 | | R: AGTCGGCCACTTCACATACG |
| | Pyruvate kinase L/R (pklr) | F: TCCTGGAGCATCTGTGTCTG | NM_201289.1 | 144 | | R: GTCTGGCGATGTTCATTCTT |
| | Phosphoenolpyruvate carboxykinase 2 (pck2) | F: TGCCTGGATGAAATTTGACA | NM_213192.1 | 106 | | R: GGCATGAGGGTTGGTTTTTA |
| | Glucose-6-phosphatase catalytic subunit 1a, tandem duplicate 1 (g6pca.1) | F: GCTCATTTCCCACACCAAGT | NM_001316336.1 | 150 | | R: ATAAAAGCCCACAGCGAATG |
| | Sterol regulatory element binding transcription factor 1 (srebf1) | F: ATGGCGGAAGACAGCAAG | NM_001105129.1 | 107 | | R: AGCGGGTTAAAGGACAGAA |
| | Sterol regulatory element binding transcription factor 2 (srebf2) | F: CACACTCTTCTCTCTGCCCG | NM_001089466.1 | 165 | | R: GATGTCGGTGAGTGAAGGGG |
| | Fatty acid synthase (fasn) | F: GGAGCAGGCTGCCTCTGTGC | XM_009306807.3 | 128 | | R: TTGCGGCCTGTCCCACTCCT |
| | Diacylglycerol O-acyltransferase 1a (dgat1a) | F: CCAAAGCTCGAACCCTGTCT | NM_199730.1 | 104 | | R: GTGTGTGAGGTTTCCCGGAT |
| | Tumor necrosis factor a (tnf-α) | F: GGGCAATCAACAAGATGGAAG | NM_212859.2 | 250 | | R: GCAGCTGATGTGCAAAGACAC |
| | Interleukin 1, beta (il1-β) | F: CGCCCTGAACAGAATGAAGCAC | NM_212844.2 | 128 | | R: AAGACGGCACTGAATCCACCAC |
| | Interleukin 6 (il-6) | F: CACGGAAAGATGTCTAACGCGAAT | NM_001261449.1 | 141 | | R: TTTATGGCCTCCAGCAGTCGTTT |
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