Effects of Dietary Fish Meal Replaced by Fish Steak Meal on Growth Performance, Antioxidant Capacity, Intestinal Health and Microflora, Inflammatory Response, and Protein Metabolism of Large Yellow Croaker Larimichthys crocea

Table 3

Primers used for real-time quantitative PCR.

Genes^1	Forward primer (5^′–3^′)	Reverse primer (5^′–3^′)	Accession number
claudin-11	ACCTCCGCCATCAAGCA	TGGGACAAAGAGCCACATC	XM_010732201.3
il-10	AGTCGGTTACTTTCTGTGGTG	TGTATGACGCAATATGGTCTG	XM_010738826.3
il-1β	CAGCTGTTCTCAAGTATGTGGC	GTTGTAAATAGTGGGTGTGTCG	XM_010736551.3
il4-13a	TGGTACTGCTGGTCAATCCG	TTTTGCCTTCAGCCAGATGT	KU885454
il4-13b	AGTTCTTCTGTCGCGCTGAG	GCTATGTATGTGCGGTTGCTG	KU885453
il-6	GCTGTTCTCAAGTATGTGGCG	TGTTGTAAATAGTGGGTGTGTCG	XM_010734753.3
occludin	AGGCTACGGCAACAGTTATG	GTGGGTCCACAAAGCAGTAA	XM_010734512.3
pcna	AGTTTGCCCGTATCTGCC	CTCTTTGTCTACATTGCTGGTCT	XM_010734227.3
tgfβ	AGCAACCACCGTACATCCTG	AGGTATCCCGTTGGCTTGTG	XM_027280465.1
tnfα	ACACCTCTCAGCCACAGGAT	CCGTGTCCCACTCCATAGTT	XM_010745990
zo-1	TGTCAAGTCCCGCAAAAATG	CAACTTGCCCTTTGACCTCT	XM_010731239.3
zo-2	ACCCGACCTGTTTGTTATTG	ATGCCGTGCTTGCTGTC	XM_027276911.1
β-actin	GACCTGACAGACTACCTCATG	AGTTGAAGGTGGTCTCGTGGA	GU584189

¹il-10, Interleukin-10; il-1β, interleukin-1β; il4-13a, interleukin-4-13a; il4-13b, interleukin-4-13b; il-6, interleukin-6; pcna, proliferating cell nuclear antigen; tgfβ, transforming growth factor β; tnfα: tumor necrosis factor α; zo-1, zonula occludens-1; nd zo-2, zonula occludens-2.