Effects of Dietary Fish Meal Replaced by Fish Steak Meal on Growth Performance, Antioxidant Capacity, Intestinal Health and Microflora, Inflammatory Response, and Protein Metabolism of Large Yellow Croaker Larimichthys crocea

Table 5

The digestive enzymes activity and oxidative stress-related parameters in the liver of large yellow croaker fed the different experimental diets (mean ± SD, n = 4)¹.

Index^2	Diets					P value
	FSM0	FSM25	FSM50	FSM75	FSM100	ANOVA	Linear	Quadratic
AMS (U/mg pro)	1.22 ± 0.08^a	1.20 ± 0.06^ab	1.10 ± 0.06^b	0.95 ± 0.05^c	0.86 ± 0.05^c	0.023	0.001	0.003
LPS (U/g pro)	50.58 ± 1.81^a	49.60 ± 3.26^a	42.46 ± 2.86^b	39.13 ± 1.64^bc	34.95 ± 2.63^c	<0.001	<0.001	<0.001
Trypsin (U/mg pro)	98.80 ± 2.03^a	96.42 ± 7.40^ab	88.73 ± 1.27^bc	85.60 ± 4.44^c	84.54 ± 3.66^c	0.007	<0.001	0.001
CAT (U/mg pro)	70.15 ± 1.85^a	70.61 ± 3.52^a	65.33 ± 4.87^a	57.64 ± 4.18^b	57.25 ± 3.04^b	0.002	<0.001	<0.001
T-SOD (U/mg pro)	120.36 ± 9.61^a	77.81 ± 10.28^b b	71.04 ± 9.98^bc	65.42 ± 6.54^bc	59.93 ± 4.58^c	<0.001	<0.001	<0.001
T-AOC (U/mg pro)	0.50 ± 0.07^a	0.45 ± 0.05^ab	0.40 ± 0.02^b	0.32 ± 0.03^c	0.30 ± 0.03^c	0.001	<0.001	<0.001
MDA (nmol/mg pro)	3.88 ± 0.30^b	4.02 ± 0.39^b	4.18 ± 0.13^b	5.47 ± 0.41^a	6.07 ± 0.36^a	<0.001	<0.001	<0.001

¹Means in the same row sharing the same superscript letter are not significantly different. The significance threshold was . ²AMS, α-amylase; CAT, catalase; FSM, fish steak meal; LPS, lipase; MDA, malonyldialdehyde; T-AOC, total antioxidant capacity; and T-SOD, total superoxide dismutase.