Dietary Sodium Butyrate Changed Intestinal Histology and Microbiota of Rainbow Trout (Oncorhynchus mykiss), but Did Not Promote Growth and Nutrient Utilization

Table 1

The formulation and proximate composition of experimental diets (air dry basis, g/kg).

Ingredients	NaB supplementation in high fishmeal diet			NaB supplementation in low fishmeal diet
	0.0	1.0	2.0	0.0	1.0	2.0
Fish meal	200.0	200.0	200.0	100.0	100.0	100.0
Soy bean meal	50.0	50.0	50.0	225.0	225.0	225.0
Soy protein concentrate	100.0	100.0	100.0	100.0	100.0	100.0
Wheat flour	259.5	258.5	257.5	175.9	174.9	173.9
Corn gluten meal	50.0	50.0	50.0	50.0	50.0	50.0
Cottonseed protein concentrate	100.0	100.0	100.0	100.0	100.0	100.0
Meat and bone meal	90.0	90.0	90.0	90.0	90.0	90.0
Brewers dried yeast	50.0	50.0	50.0	50.0.	50.0	50.0
Fish oil	30.0	30.0	30.0	37.0	37.0	37.0
Soybean oil	30.0	30.0	30.0	30.0	30.0	30.0
DL-methionine	0.0	0.0	0.0	0.9	0.9	0.9
L-Lysine	0.0	0.0	0.0	0.7	0.7	0.7
Vitamin premix^a	10.0	10.0	10.0	10.0	10.0	10.0
Mineral premix^b	30.0	30.0	30.0	30.0	30.0	30.0
Y2O3	0.5	0.5	0.5	0.5	0.5	0.5
Total	1000.0	1000.0	1000.0	1000.0	1000.0	1000.0
Proximate composition
Crude protein	441.9	437.5	438.6	434.0	435.8	436.0
Crude lipid	85.4	81.5	77.9	82.0	82.2	81.6
Crude ash	133.9	135.1	134.2	128.6	130.8	131.1
Moisture	63.6	65.2	60.3	62.1	63.7	60.9

^aVitamin premix (mg or IU/kg diet): VA, 10000IU; VD₃, 3000IU; VE, 150IU; VK₃, 12.17 mg; VB₁, 20 mg; VB₂, 20 mg; VB₃, 100 mg; VB₆, 22 mg; VB₁₂, 0.15 mg; VC, 1000 mg; biotin, 0.6 mg; folic acid, 8 mg; inositol, 500 mg. ^bMineral premix (mg/kg diet): iodine, 1.5 mg; cobalt, 0.6 mg; copper, 3 mg; iron, 63 mg; zinc, 89 mg; manganese, 11.45 mg; selenium, 0.24 mg; magnesium, 180 mg.