Effects of Apple Polyphenols and Taurine on Growth Performance, Tissue Morphology, and Lipid and Glucose Metabolism in Rice Field Eel (Monopterus albus) Fed High Oxidized Fish Oil
Table 2
The primers of RT-PCR used in the experiment.
Target gene
Forward sequences (5′–3′)
Reverse sequences (5′–3′)
Size (bp)
Accession number
ampkα1
TTGAGTGTGCAGAGGAGGAGGTC
TGAGGTTAGGTGCTGGTCGTCTAG
176
XM_020619494.1
accα
GTTCCAACCAAGGCTCCGTATGAC
TCCACAGCAACCACTCCAGTAGG
191
XM_020598745.1
cpt1
CCTGGAAGAAGCGTGTCATCAGAC
TGACTGGCAGGTGCTCCTGTATC
168
XM_020625222.1
pparα
TTGCCATGCTTGCCTCCAGTATG
GCCATGTCACTGTCGTCCAACTC
175
XM_020621872.1
lipin
GCCGCCACCTTGGTTCTGATG
CTGTCCACTCCGCTGTCTCCTC
181
XM_020623677.1
pck1
AGACTACACCACCATGCCTCCTG
TTCTCCTCGTCAGAGCCGTCAC
171
XM_020621224.1
gsk3α
GTGCCACCGACTACACATCCAAC
TGGTCCACACCGCTGTCTCC
106
XM_020610802.1
ef-1α
CGCTGCTGTTTCCTTCGTCC
TTGCGTTCAATCTTCCATCCC
202
XM_020588923.1
β-actin
GCGTGACATCAAGGAGAAGC
CTCTGGGCAACGGAACCTCT
156
AY345056
Note: ampkα1 = AMP-activated protein kinase α1; accα = phosphorylate of acetyl-coenzyme A (CoA) carboxylase; cpt1 = carnitine palmitoyltransferase 1; pparα = peroxisome proliferator-activated receptor α; pck1 = phosphoenolpyruvate carboxykinase 1; and gsk3α = glycogen synthase kinase 3α.
