Optimizing Artemia Enrichment: A Low DHA/High EPA Protocol for Enhanced n-3 LC-HUFA Levels to Support Greater Amberjack (Seriola dumerili) Larval Rearing

Table 1

Experimental emulsion ingredients (g kg⁻¹) and selected fatty acid contents (percentage of total fatty acids, % TFA) of total lipids in the experimental emulsions.

	EPA-0	EPA-1			EPA-2	EPA-3			EPA-4
Ingredients (g kg^−1 diet)
Incromega EPA 500TG	0	300			450	600			900
Oleic acid (OA)	900	600			450	300			0
Soybean lecithin	94.5	94.5			94.5	94.5			94.5
DL-α-tocopherol acetate	3	3			3	3			3
Vitamin C	2.5	2.5			2.5	2.5			2.5
Fatty acid content (% TFA)
Saturated	15.21	12.66			11.46	9.27			2.61
Monoenoics	74.63	58.63			50.00	36.95			7.60
n-3	2.16	20.48			30.11	45.09			79.52
n-6	7.93	7.43			7.16	6.95			7.65
n-9	72.17	56.07			47.47	34.57			5.56
Total n-3 LC-HUFA	1.37	17.35			25.60	38.74			70.62
14 : 0	0.02	0.12			0.20	0.27			0.35
16 : 0	11.45	9.21			7.99	6.01			0.95
16 : 1n-7	0.76	0.81			0.88	0.89			0.87
18 : 0	3.14	2.54			2.25	1.72			0.41
18 : 1n-9 (OA)	72.13	55.84			47.13	34.06			3.94
18 : 1n-7	1.40	1.46			1.33	1.16			0.94
18 : 2n-6 (LA)	7.80	6.14			5.16	4.05			1.96
18 : 3n-3 (ALA)	0.67	0.65			0.64	0.66			1.18
20 : 1n-9	0.02	0.01			0.01	0.02			0.41
20 : 4n-6 (ARA)	0.06	0.82			1.25	1.83			3.53
20 : 5n-3 (EPA)	0.84	14.28			21.18	31.97			60.16
22 : 6n-3 (DHA)	0.30	2.36			3.34	5.11			6.79
ARA/EPA	0.07	0.06			0.06	0.06			0.06
DHA/EPA	0.36	0.17			0.16	0.16			0.11
DHA/ARA	5.41	2.89			2.68	2.80			1.92
OA/DHA	240.20	23.67			14.13	6.66			0.58
OA/n-3 LC-HUFA	52.71	3.22			1.84	0.88			0.06
n-3/n-6	0.27	2.76			4.21	6.49			10.40