Aquaculture Nutrition

Research Article

The Assessment of Different Dietary Selenium Resources on Reproductive Performance, Spawning Indicators, and Larval Production of Red Tilapia (Oreochromis mossambicus × O. niloticus) Broodfish

Table 1

Ingredient and proximate composition (g/1,000 g dry diet) of the red tilapia brooders diets.


Ingredient (g/kg)	CTR	Na₂SeO₃	NPSe⁺	Na₂SeO₃ + NPSe

Soybean meal	102.4	102.4	102.4	102.4
Fish meal	38.6	38.6	38.6	38.6
Casein	226.1	226.1	226.1	226.1
Gelatin	60	60	60	60
Fish oil	45.2	45.2	45.2	45.2
Crude palm oil	46.3	46.3	46.3	46.3
Corn starch	247.6	247.6	247.6	247.6
Vitamin mix^a	30	30	30	30
Ascorbic acid	5	5	5	5
Mineral mix^b	20	20	20	20
Di calcium phosphate	10	10	10	10
Carboxyl methylcellulose	20	20	20	20
α-Cellulose	148.8	148.8	148.8	148.8
Total	1,000	1,000	1,000	1,000
Se supplementation
Na₂SeO₃	0.0	0.001	0.0	0.0005
NPSe	0.0	0.0	0.001	0.0005
Proximate composition
Moisture	12.5	12.5	12.5	12.5
Crude protein	35.91	35.91	35.91	35.91
Crude lipids	10.2	10.2	10.2	10.2
Ash	4	4	4	4
Fiber	6.4	6.4	6.4	6.4
NFE^c	43.49	43.49	43.49	43.49
Analyzed dietary Se levels (mg Se/kg)	0.59	1.38	1.49	1.44

Na₂SeO₃, sodium selenite;⁺NPSe, selenium nanoparticles. ^aFormulation (g/kg): α-tocopheryl acetate, 2; inositol, 5; choline bitartrate, 136.06; niacin, 4.5; riboflavin, 1; pyridoxine·HCl, 1; thiamin·HCl 0.92; D calcium pantothenate, 3; retinyl acetate, 0.6; cholecalciferol, 0.083; menadione 1.67; D-biotin, 0.02; folic acid, 0.09; vitamin B12, 0.00135; and cellulose, 834.167. ^bFormulation (g/kg): calcium phosphate monobasic, 135.5; calcium L-lactate hydrate, 327.0; ferric citrate, 29.7; magnesium sulfate·7H₂O, 132.0; potassium phosphate dibasic, 239.8; sodium phosphate monobasic·H₂O, 87.2; sodium chloride, 43.5; potassium iodide, 0.15; cuprous chloride, 0.2; manganous sulfate·H₂O, 0.8; cobalt chloride·6H₂O, 1.0; zinc sulfate·7H₂O, 3.0; and sodium selenite, 0.011. ^cNitrogen-free extract (NFE) = 100 − (protein + lipid + ash + fiber).