The Assessment of Different Dietary Selenium Resources on Reproductive Performance, Spawning Indicators, and Larval Production of Red Tilapia (Oreochromis mossambicus × O. niloticus) Broodfish

Table 4

Egg biometric indices and fry measurements of adult female red tilapia fish fed diets supplemented with various selenium sources over 25 weeks.

Measurements	Experimental groups				P Value
	CTR	Na2SeO3	NPSe	Na2SeO3 + NPSe
Egg diameter^a (µm)	20.76 ± 0.68^c	23.9 ± 0.36^b	26.23 ± 0.25^a	25.8 ± 0.10^a	<0.001
Mean egg volume^b (mm^3)	5.47 ± 0.41^d	6.30 ± 0.75^c	6.92 ± 0.68^a	6.80 ± 0.29^a,b	<0.001
Mean egg wet weight (mm)	4.85 ± 0.11^c	5.59 ± 0.38^b,c	6.14 ± 0.24^a	6.03 ± 0.47^a,b	<0.001
Mean fry number	792.6 ± 11.02^d	1016 ± 29.46^c	1,324.3 ± 21.36^a	1,256.3 ± 51.82^b	<0.001
Mean fry weight (g)	10.83 ± 0.15^d	13.93 ± 0.06^c	16.8 ± 0.26^a	15.6 ± 0.66^b	<0.001

CTR, control group fed unsupplemented diet; Na₂SeO₃, fish group administered diet supplemented with sodium selenite (1 mg Se/kg); NPSe, fish group administered diet supplemented with selenium nanoparticles (1 mg NPSe/kg); Na₂SeO₃ + NPSe, fish group administered diet supplemented with combination of sodium selenite (0.5 mg Se) and selenium nanoparticles (0.5 mg NPSe) per kg. ^aMean egg diameter (µm) = (long-axis length + short-axis length)/2. ^bMean egg volume (mm³) = π/6 × long axis × short axis². The presented data were analyzed by applying one-way ANOVA. Data are the mean ± SD. The significant between represented values was identified at . ^a,b,c,dDifferent lowercase superscript letters in the same row indicate that the difference between groups is significant.