High Levels of Vitamin A in Plant-Based Diets for Gilthead Seabream (<i>Sparus aurata</i>) Juveniles, Effects on Growth, Skeletal Anomalies, Bone Molecular Markers, and Histological Morphology

<table class="table-group" id="tab2"><tr><td><table class="table"><tr><td class="thead-hr" colspan="3"><hr/></td></tr><tr class="thead"><td class="align_left">Gene</td><td class="align_center">Nucleotide sequence (5<sup>′</sup>–3<sup>′</sup>)</td><td class="align_center">Accession number</td></tr><tr><td class="thead-hr" colspan="3"><hr/></td></tr><tr><td class="align_left" rowspan="2">Beta-actin (<i>bact</i>)</td><td class="align_center">F: TCTGTCTGGATCGGAGGCTC</td><td class="align_center" rowspan="2">X89920</td></tr><tr><td class="align_center">R: AAGCATTTGCGGTGGACG</td></tr><tr><td class="align_center" colspan="3"><hr/></td></tr><tr><td class="align_left" rowspan="2">Elongation factor 1-<i>α</i> (<i>ef1α</i>)</td><td class="align_center">F: CATGGTTGTGGAGCCCTTCT</td><td class="align_center" rowspan="2">AF184170</td></tr><tr><td class="align_center">R: TCCTGCACGACCATTCATTTC</td></tr><tr><td class="align_center" colspan="3"><hr/></td></tr><tr><td class="align_left" rowspan="2">Alkaline phosphatase (<i>alp</i>)</td><td class="align_center">F: AGAACGCCCTGACGCTGCAA</td><td class="align_center" rowspan="2">AY266359</td></tr><tr><td class="align_center">R: TTCAGTATACGAGCAGCCGTCAC</td></tr><tr><td class="align_center" colspan="3"><hr/></td></tr><tr><td class="align_left" rowspan="2">Runx-related transcription factor 2 (<i>runx2</i>)</td><td class="align_center">F: GCCTGTCGCCTTTAAGGTGGTTGC</td><td class="align_center" rowspan="2">AJ619023</td></tr><tr><td class="align_center">R: TCGTCGTTGCCCGCCATAGCTG</td></tr><tr><td class="align_center" colspan="3"><hr/></td></tr><tr><td class="align_left" rowspan="2">Osteocalcin (<i>oc</i>)</td><td class="align_center">F: GGCAGCCATCTGTCTGACTT</td><td class="align_center" rowspan="2">AF048703.1</td></tr><tr><td class="align_center">R: GGTCCGTAGTAGGCCGTGTA</td></tr><tr><td class="align_center" colspan="3"><hr/></td></tr><tr><td class="align_left" rowspan="2">Bone morphogenic protein 2 (<i>bmp2</i>)</td><td class="align_center">F: GTGGCTTCCATCGTATCAACATTTT</td><td class="align_center" rowspan="2">JF261172.1</td></tr><tr><td class="align_center">R: GCTCCCCGCCATGAGT</td></tr><tr class="table-tr"><td colspan="3"><hr class="tbody-hr"/></td></tr></table></td></tr></table>

<div>Sequences of primers used for gene expression analyses.</div>

Aquaculture Nutrition

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Table 2

Table 2: High Levels of Vitamin A in Plant-Based Diets for Gilthead Seabream (<i>Sparus aurata</i>) Juveniles, Effects on Growth, Skeletal Anomalies, Bone Molecular Markers, and Histological Morphology 

Table 2 | High Levels of Vitamin A in Plant-Based Diets for Gilthead Seabream (Sparus aurata) Juveniles, Effects on Growth, Skeletal Anomalies, Bone Molecular Markers, and Histological Morphology 