Research Article
Compared to Fishmeal, Dietary Soybean Meal Improves the Reproductive Performance of Female Yellow Catfish (Pelteobagrus fulvidraco) Broodstock
Table 4
Primers used in quantitative real-time PCR analysis.
| | Gene | GeneBank accession no. | Forward primer (5-3) | Revers primer (5-3) |
| | β-Actin | XM027148463.1 | TTGGCAATGAGAGGTTCAGGT | TGCCAGGGTACATGGTGGTA | | ef1a | XM027175544.2 | TTAAAGGTGGTGGGGCCTTG | CTGTGGCAACAGGTGCAGAT | | Sf-1 | XM027148774.2 | GTGCGTGTGTCTGTTTGGAC | CTGACCCTTTACTTTGCGCC | | erβ | KT832703.1 | GCCTCTCAGTCCGTCGTTCA | GTGCTAGGTGGCTTGCTGTC | | StAR | KP707128.1 | GCAGACGATCCCAACAAGAC | CCGTTGCCTCCATTCTGATG | | 3β-hsd | KP701031 | AGTTCCACAAAACACCGAGC | TACACTTCACCTCCGAGTCG | | 17β-hsd | XM027161133.2 | GAAAAGCATCTCACCTCCCG | AGCCAGTGATCAGAACGACC | | 20β-hsd | FJ465136.1 | GTGTCGTGCTCTGCTCCCA | TTCCGCTCCTTCCTCTGT | | cyp11a1 | KP135444.1 | ATGGTGAACGTCTGGGGCT | TCGTAACATTGAAGGGGGG | | cyp17a1 | HQ83264.3 | TACCTGGAAGCCACAATC | TCATTCAGGAAACGCTCT |
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Abbreviations: β-actin: beta-actin; ef1α: elongation factor 1-alpha; sf-1: steroidogenic factor 1; erβ: estrogen receptor beta; StAR: steroidogenic acute regulatory protein; 3β-hsd: 3beta-hydroxysteroid dehydrogenase; 17β-hsd: 17beta-hydroxysteroid dehydrogenase; 20β-hsd: 20beta-hydroxysteroid dehydrogenase; cyp11a1: cytochrome P450 11a1; cyp17a1: cytochrome P450 17a1.
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