Dietary Epigallocatechin-3-Gallate (EGCG) Improves Nonspecific Immune Response of Chinese Rice Field Eel (Monopterus albus)

Table 1

Formulation and proximate composition of the experimental diets (g/kg, dry matter basis).

Feed composition (g/kg)	Control	EGCG25	EGCG50	EGCG100	EGCG200	EGCG400
Fish meal	450.00	450.00	450.00	450.00	450.00	450.00
Soybean meal	150.00	150.00	150.00	150.00	150.00	150.00
Wheat meal	80.00	80.00	80.00	80.00	80.00	80.00
α-Starch	160.00	160.00	160.00	160.00	160.00	160.00
Blood meal	20.00	20.00	20.00	20.00	20.00	20.00
Brewer yeast	50.00	50.00	50.00	50.00	50.00	50.00
Fish oil	40.00	40.00	40.00	40.00	40.00	40.00
Ca(H2PO4)2	15.00	15.00	15.00	15.00	15.00	15.00
Premix^a	10.00	10.00	10.00	10.00	10.00	10.00
Choline	5.00	5.00	5.00	5.00	5.00	5.00
Earthworm meal	10.00	10.00	10.00	10.00	10.00	10.00
Mold inhibitor^b	3.00	3.00	3.00	3.00	3.00	3.00
Sodium carboxymethyl cellulose	2.00	2.00	2.00	2.00	2.00	2.00
Microcrystalline cellulose	5.00	4.975	4.95	4.90	4.80	4.60
EGCG	0.00	0.025	0.050	0.10	0.20	0.40
Proximate composition
Crude protein	429.80	429.40	429.80	433.50	430.30	432.40
Crude lipid	79.30	79.80	8.00	79.90	80.50	80.90
EGCG	0.00	0.029	0.052	0.075	0.17	0.35

Note. ^aVitamin and mineral premix composition (mg/kg): NaCl 50 mg, KI 1.5 mg, CoCl₂ · 6H₂O 2.5 mg, CuSO₄ · 5H₂O 15 mg, FeSO₄·H₂O 1,250 mg, ZnSO₄·H₂O 175 mg, MnSO₄·H₂O 80 mg, Na₂SeO₃·5H₂O 1.0 mg, MgSO₄·H₂O 1,500 mg, vitamin B₁ 12 mg, riboflavin 12 mg, vitamin B₆ 8 mg, vitamin B₁₂ 0.05 mg, vitamin K₃ 8 mg, inositol 100 mg, pantothenic acid 40 mg, niacin acid 50 mg, folic acid 5 mg, biotin 0.8 mg, vitamin A 25 mg, vitamin D₃ 35 mg, vitamin E 50 mg, and vitamin C 100 mg. ^bMold inhibitor: sodium benzyl alcohol 40%, gluconolactone 30%, sodium dehydroacetate 10%, sorbic acid methyl 10%, and disodium EDTA 10%.