Dietary Cholesterol Requirements of Large Red Swamp Crayfish (Procambarus clarkii)

Table 2

Effect of dietary cholesterol level on the growth performance and feed utilization rate of P. clarkii.

Dietary cholesterol level (g/kg)

Parameters

IBW^1 (g)

FBW^2 (g)

WGR^3 (%)

SGR^4 (%/d)

FCR^5

SR^6 (%)

HSI^7 (%)

AFC^8 (%)

CFC^9 (%)

TFC^10 (%)

PER^11 (%)

PDR^12 (%)

2.47 (C0)

13.39 ± 0.47

21.35 ± 0.56^a

59.47 ± 2.54^a

0.83 ± 0.03^a

1.50 ± 0.04^b

88.89 ± 0.00

8.68 ± 0.59

12.41 ± 0.45

6.31 ± 0.07^b

18.72 ± 0.38^a

206.48 ± 0.64^a

23.72 ± 1.72^a

4.27 (C1)

13.45 ± 0.34

22.19 ± 0.85^ab

64.97 ± 2.26^a

0.90 ± 0.02^ab

1.43 ± 0.06^b

90.74 ± 8.49

9.22 ± 0.40

13.00 ± 0.90

6.41 ± 0.20^b

19.41 ± 1.07^a

220.10 ± 8.97^a

25.34 ± 1.54^ab

6.80 (C2)

13.53 ± 0.19

23.27 ± 0.57^bc

71.95 ± 3.82^b

0.97 ± 0.04^bc

1.18 ± 0.06^a

85.18 ± 3.21

9.29 ± 0.21

13.80 ± 0.66

7.79 ± 0.19^c

21.59 ± 0.50^b

249.64 ± 11.68^b

27.79 ± 1.88^ab

8.77 (C3)

13.51 ± 0.12

23.57 ± 0.52^c

74.43 ± 2.33^bc

0.99 ± 0.03^cd

1.15 ± 0.05^a

85.18 ± 3.21

8.84 ± 0.53

12.61 ± 0.41

6.82 ± 0.11^b

19.43 ± 0.30^a

259.39 ± 9.87^b

28.61 ± 2.21^b

11.74 (C4)

13.56 ± 0.07

24.52 ± 0.42^c

80.87 ± 3.98^c

1.06 ± 0.04^d

1.16 ± 0.03^a

88.89 ± 5.56

8.79 ± 0.83

12.52 ± 0.73

6.44 ± 0.12^b

18.96 ± 0.82^a

272.26 ± 7.15^b

28.20 ± 2.22^ab

14.24 (C5)

13.52 ± 0.08

23.33 ± 0.43^bc

72.54 ± 2.90^b

0.97 ± 0.03^bc

1.21 ± 0.08^a

79.63 ± 3.20

8.68 ± 0.26

12.08 ± 0.91

5.42 ± 0.41^a

17.50 ± 1.26^a

255.50 ± 15.07^b

27.64 ± 0.14^ab

ANOVA

P value

0.967

0.001

0.001

0.001

0.001

0.128

0.546

0.125

0.001

0.001

0.001

0.033

Regression (n = 3)

Linear

Adj. R^2

−0.015

0.506

0.543

0.534

0.509

0.183

−0.024

0.22

0.097

0.078

0.624

0.316

P value

0.401

0.001

0.001

0.001

0.001

0.043

0.447

0.256

0.112

0.138

0.001

0.009

Quadratic

Adj. R^2

−0.062

0.73

0.788

0.791

0.849

0.146

0.011

0.188

0.708

0.51

0.842

0.534

P value

0.615

0.001

0.001

0.001

0.001

0.12

0.36

0.082

0.001

0.002

0.001

0.001

Note: Values represent mean ± SD. Different letters in the same row indicate a significant difference between groups (). C0–C5 represent each group. N_t = the final mantissa; N₀ = the initial mantissa; W_t = final body weight (g); W₀ = the initial body weight (g); t = the experimental days (d); W_f = feed intake (g); W_d = the total dead crayfish weight (g); W_h = hepatopancreas weight (g); W_m = the muscle weight of shrimp tail (g); Wc = muscle weight of the first chelopod (g); P = the crude protein content of the feed (%); P_t = the final body crude protein content (%); P₀ = the initial body protein content (%). ¹IBW (g): initial mean weight. ²FBW (g): final mean weight. ³WGR (weight gain rate, %) = (W_t − W₀)/W₀ × 100. ⁴SGR (specific growth rate, %/d) = (lnW_t − lnW₀)/t × 100. ⁵FCR (feed conversion ratio) = W_f/(W_tN_t − W₀N₀ + W_d). ⁶SR (survival rate, %) = N_t/N₀ × 100. ⁷HSI (hepatosomatic index, %) = W_h/W_t × 100. ⁸AFC (abdomen flesh percentage, %) = W_m/W_t × 100. ⁹CFC (clawfoot flesh percentage, %) = W_c/W_t × 100. ¹⁰TFC (total flesh percentage, %) = (W_m + W_c)/W_t × 100. ¹¹PER (protein efficiency ratio, %) = (W_t − W₀)/(W_f × P) × 100. ¹²PDR (protein deposition rate, %) = (W_t × P_t − W₀ × P₀)/(W_f × W_p) × 100.