Dietary Cholesterol Requirements of Large Red Swamp Crayfish (Procambarus clarkii)

Table 6

Effect of cholesterol level on the hepatopancreas and intestinal digestive enzyme activities of P. clarkii.

Dietary cholesterol level (g/kg)	Hepatopancreas			Intestine
	Protease (U/mg)	Lipase (U/g prot)	Amylase (U/mg prot)	Protease (U/mg)	Lipase (U/g prot)	Amylase (U/mg prot)
2.47 (C0)	72.56 ± 1.15	15.47 ± 0.19^a	0.16 ± 0.03^ab	26.33 ± 1.28^ab	9.96 ± 0.37^a	0.86 ± 0.02^a
4.27 (C1)	70.22 ± 1.24	15.70 ± 0.36^ab	0.14 ± 0.02^a	27.61 ± 1.63^ab	10.96 ± 0.85^ab	1.37 ± 0.11^b
6.80 (C2)	70.88 ± 2.35	16.07 ± 0.33^bc	0.15 ± 0.01^a	29.24 ± 1.81^b	11.41 ± 0.52^b	2.35 ± 0.14^c
8.77 (C3)	72.16 ± 1.40	16.39 ± 0.07^cd	0.20 ± 0.01^bc	34.62 ± 1.32^c	11.60 ± 0.13^b	2.89 ± 0.06^d
11.74 (C4)	70.29 ± 3.46	16.83 ± 0.05^d	0.24 ± 0.02^c	26.18 ± 1.27^ab	11.77 ± 0.44^b	2.86 ± 0.08^d
14.24 (C5)	72.69 ± 1.67	16.82 ± 0.12^d	0.22 ± 0.01^c	24.75 ± 1.41^a	11.95 ± 0.33^b	2.84 ± 0.16^d
ANOVA
P value	0.334	0.001	0.001	0.001	0.001	0.001
Regression (n = 3)
Linear
Adj. R^2	−0.041	0.859	0.582	−0.053	0.648	0.81
P value	0.774	0.001	0.001	0.702	0.001	0.001
Quadratic
Adj. R^2	−0.015	0.855	0.573	0.431	0.73	0.952
P value	0.45	0.003	0.001	0.006	0.001	0.001

Note: Values represent mean ± SD. Different letters in the same row indicate a significant difference between groups ().