Physiological and Neurobehavioral Disturbances Induced by Al₂O₃ Nanoparticle Intoxication in Nile Tilapia Fish: Benefits of Dietary Chamomile Essential Oil

Table 2

Formulation and calculated composition analysis of the basal diet fed to experimental O. niloticus fish.

Items	Control
Ingredient (%)
Yellow corn	210
Soybean meal 48% CP	200
Fish meal	150
Corn gluten 60% CP	130
Rice bran	110
Wheat middlings	150
Premix-min	10
Premix-vit	10
Corn oil	30
Total	1000
Calculated composition
Crude protein (%)	320.5
Lipid (%)	45.50
Crude fiber (%)	42.45
Ash (%)	73.01
Nitrogen-free extract	518.54

Composition of mineral premix kg⁻¹: manganese, 53 g; zinc, 40 g; iron, 20 g; copper, 2.7 g; iodine, 0.34 g; selenium, 70 mg; cobalt, 70 mg; and calcium carbonate as a carrier up to 1 kg. Composition of vitamin premix kg⁻¹: vitamin A, 8,000,000 IU; vitamin D3, 2,000,000 IU; vitamin E, 7,000 mg; vitamin K3, 1,500 mg; vitamin B1, 700 mg; vitamin B2, 3,500 mg; vitamin B6, 1,000 mg; vitamin B12, 7 mg; biotin, 50 mg; folic acid, 700 mg; nicotinic, 20,000 mg; and pantothenic acid, 7,000 mg. Nitrogen-free .