Pot Marigold (Calendula officinalis) Powder in Rainbow Trout (Oncorhynchus mykiss) Feed: Effects on Growth, Immunity, and Yersinia ruckeri Resistance

Table 2

Ingredients and proximate compositions of different trial diets.

Ingredients	Control	MP 0.5%	MP 1%	MP 1.5%	MP 2%
Fish meal^a (g/kg)	495	495	495	495	495
Soybean meal ^b(g/kg)	127	127	127	127	127
Wheat flour (g/kg)	150	150	150	150	150
Corn gluten meal^c (g/kg)	70	70	70	70	70
Fish oil (g/kg)	45	45	45	45	45
Soybean oil (g/kg)	35	35	35	35	35
Mineral mix^d (g/kg)	10	10	10	10	10
Vitamins mix^d (g/kg)	10	10	10	10	10
Lecithin^e (g/kg)	0.5	0.5	0.5	0.5	0.5
L-Threonine (g/kg)	0.4	0.4	0.4	0.4	0.4
DL-methionine (g/kg)	0.1	0.1	0.1	0.1	0.1
Mono calcium phosphate (g/kg)	5	5	5	5	5
Cane molasses (g/kg)	20	20	20	20	20
Antioxidant (BHT)^f (g/kg)	0.2	0.2	0.2	0.2	0.2
Toxin binder (antimycotoxin) (g/kg)	1.8	1.8	1.8	1.8	1.8
Filler (g/kg)	30	25	20	15	10
Marigold powder (MP) (g/kg)	0	5	10	15	20
Proximate composition% in dry basis
Crude protein (%)	45.18	45.10	44.88	44.60	44.50
Crude lipid (%)	14.14	14.10	14.00	13.95	13.90
Crude ash (%)	9.20	9.26	9.32	9.30	9.37
Crude carbohydrate (%)	22.5	22.76	23.3	23.77	24.09
Dry matter (%)	91.02	91.22	91.50	91.62	91.86
Gross energy (kcal/g)	487.60	487.84	487.87	487.74	488.02

^aZafar Fishmeal Factory, Guilan, Iran (crude protein 66.10%). ^bSoybean Co., Gorgan, Iran (crude protein 45.2%). ^cGlucosan Co., Qazvin, Iran. ^dThe premix provided the following amounts per kilogram of feed: A: 1000 IU; D3: 5000 IU; E: 20 mg; B5: 100 mg; B2: 20 mg; B6: 20 mg; B1: 20 mg; H: 1 mg; B9: 6 mg; B12: 1 mg; B4: 600 mg; C:50 mg; Mg: 350 mg; Fe: 13 mg; Co: 2.5 mg; Cu: 3 mg; Zn: 60 mg; Se: 0.3 mg; I: 1.5 mg; and Mn: 10 mg. Chinechin Co., Tehran, Iran. ^ePouyavision, Tehran, Iran. ^fButylated hydroxytoluene (BHT) (Merck, Germany).