Cottonseed Meal Protein Hydrolysate Improves the Growth Performance of Chinese Mitten Crab (Eriocheir sinensis) by Promoting the Muscle Growth and Molting Performance

Table 2

Formulation and proximate composition of the experimental diets.

Ingredients (%)	CPH0	CPH0.2	CPH0.4	CPH0.8	CPH1.6	CPH3.2
Fish meal	30.00	30.00	30.00	30.00	30.00	30.00
Blood meal	4.00	4.00	4.00	4.00	4.00	4.00
Soybean meal (defatted)	10.00	10.00	10.00	10.00	10.00	10.00
Cotton meal	4.00	3.78	3.56	3.12	2.24	0.47
CPH	0	0.20	0.40	0.80	1.60	3.20
Peanut meal	18.81	18.81	18.81	18.81	18.81	18.81
Rapeseed meal	2.00	2.00	2.00	2.00	2.00	2.00
α-Starch	20.93	20.95	20.97	21.01	21.10	21.26
Soybean oil	3.55	3.55	3.55	3.55	3.55	3.55
Fish oil	1.00	1.00	1.00	1.00	1.00	1.00
Ca(H2PO4)2	1.50	1.50	1.50	1.50	1.50	1.50
Zeolite powder	0.9	0.9	0.9	0.9	0.9	0.9
Premix^a	1.00	1.00	1.00	1.00	1.00	1.00
Mixture^b	2.30	2.30	2.30	2.30	2.30	2.30
Proximate composition (g/kg, dry-matter basis)
Dry matter	89.46	89.55	89.36	89.94	89.79	90.36
Crude protein	39.78	39.85	39.91	39.87	39.79	39.78
Crude lipid	7.51	7.49	7.48	7.50	7.49	7.46

^aPremix supplied the following minerals (g/kg) and vitamins (IU or mg/kg) per kilogram: CuSO₄·5H₂O, 2.0 g; FeSO₄·7H₂O, 25 g; ZnSO₄ · 7H₂O, 22 g; MnSO₄·4H₂O, 7 g; Na₂SeO₃, 0.04 g; KI, 0.026 g; CoCl₂·6H₂O, 0.1 g; vitamin A, 900,000 IU; vitamin D, 200,000 IU; vitamin E, 4,500 mg; vitamin K₃, 220 mg; vitamin B₁, 320 mg; vitamin B₂, 1,090 mg; vitamin B₅, 2,000 mg; vitamin B₆, 500 mg; vitamin B₁₂, 1.6 mg; vitamin C, 10,000 mg; pantothenate, 1,000 mg; folic acid, 165 mg; choline, 60,000 mg; biotin, 100 mg; and Myo-inositol 15,000 mg. ^bMixture includes the following ingredients (%): choline chloride 4.21%; antioxidants 1.26%; mildew-proof agent 2.09%; salt 21.03%; lvkangyuan 63.15%; and biostimep 8.26%.CPH, cottonseed meal protein hydrolysate; CPH0 to CPH3.2, dietary cotton meal replacement by 0%, 0.2%, 0.4%, 0.8%, 1.6%, and 3.2% of CPH.