Effect of Moringa oleifera, Bacillus amyloliquefaciens, and Their Combination on Growth Performance, Digestive Enzymes, Immunity, and Microbiota in Nile Tilapia (Oreochromis niloticus)

Table 2

Primers used for RT-PCR analysis.

Target mRNA	Primer sequence (5^′-3^′)	Accession number
IL-6	F:ACAGAGGAGGCGGAGATG	XM_019350387.2
	R:GCAGTGCTTCGGGATAGAG	—
IL-10	F:TGGAGGGCTTCCCCGTCAG	XM_019358799.2
	R:CTGTCGGCAGAACCGTGTCC	—
TNF-α	F:TAGAAGGCAGCGACTCAA	NC_031985.2
	R:CCTGGCTGTAGACGAAGT	—
C3	F:CAGGCAGGAGGATGTATCGG	XM_005454829.4
	R:TGCCAGCGTCAAGTCTTTTCT	—
Β-actin	F:CAGGGAGAAGATGACCCAGA	XM_003443127.5
	R:CAGGGCATAACCCTAGTAGA	—