Effect of Moringa oleifera, Bacillus amyloliquefaciens, and Their Combination on Growth Performance, Digestive Enzymes, Immunity, and Microbiota in Nile Tilapia (Oreochromis niloticus)
Table 6
The digestive enzymes of Nile tilapia fed test diets for 56 days.
Treatments
CON
MO
P values
CON
Ba
CON
Ba
MO
Ba
MO × Ba
Foregut
LPS (U/gprot)
0.78 ± 0.20
3.40 ± 0.80
2.37 ± 0.33
2.64 ± 0.49
0.465
0.022 (x/y)
0.055
AMS (U/mgprot)
7.23 ± 0.85
13.78 ± 2.54
9.05 ± 1.90
15.27 ± 2.95
0.882
0.005 (x/y)
0.543
CAS (U/mgprot)
3.65 ± 0.32
2.22 ± 0.27
2.76 ± 0.99
2.73 ± 0.41
0.745
0.233
0.249
Midgut
LPS (U/gprot)
3.13 ± 0.64
2.00 ± 0.25
3.65 ± 0.68
2.93 ± 0.41
0.194
0.105
0.711
AMS (U/mgprot)
5.07 ± 0.26
5.49 ± 0.30
5.76 ± 0.67
5.24 ± 0.10
0.487
0.688
0.822
CAS (U/mgprot)
2.21 ± 0.30
2.71 ± 0.33
2.63 ± 0.59
2.57 ± 0.13
0.719
0.575
0.477
Hindgut
LPS (U/gprot)
1.73 ± 0.39
2.48 ± 0.25
1.50 ± 0.03
1.87 ± 0.07
0.108
0.045 (x/y)
0.436
AMS (U/mgprot)
4.78 ± 0.85
5.04 ± 0.27
4.88 ± 0.39
5.73 ± 0.29
0.143
0.050 (x/y)
0.206
CAS (U/mgprot)
1.54 ± 0.05
1.30 ± 0.12
1.41 ± 0.07
1.24 ± 0.14
0.406
0.068
0.736
Stomach
LPS (U/gprot)
1.84 ± 0.23
1.15 ± 0.04
2.38 ± 0.59
2.00 ± 0.07
0.180
0.963
0.931
CAS (U/mgprot)
6.26 ± 0.95
7.32 ± 0.42
5.21 ± 0.83
6.05 ± 0.70
0.009 (b/a)
0.026 (x/y)
0.777
Notes: MO, M. oleifera supplementation; Ba, B. amyloliquefaciens supplementation; MO × Ba, interaction effect. Values are means and standard error of the mean (SEM). ns, no significant, . Different letters indicate significant differences between the two MO adding types and B. amyloliquefaciens adding types, such as (a/b) in parentheses corresponds to no MO addition, MO addition, and the value of a is less than b. The other set of letters (x/y) corresponds to no B. amyloliquefaciens addition and B. amyloliquefaciens addition, and the value of x is less than the value of y.
