Effects of the Replacement of Dietary Fishmeal by the Blend of Tenebrio molitor Meal, Chlorella Meal, Clostridium Autoethanogenum Protein, and Cottonseed Protein Concentrate on Growth, Protein Utilization, and Intestinal Health of Gibel Carp (Carassius gibelio, CAS Ⅴ)

Table 3

Primer sequences were used for the analysis of mRNA expression by qRT-PCR.

Gene name	GenBank accession number	Forward primer (5^′ to 3^′)	Reverse primer (5^′ to 3^′)
β-actin	JN006052.1	TGGGACAGAAGGACAGCTATG	AGCTCGTTGTAGAAGGTGTGA
tnf-α	XM_026282152.1	TGTTCTCAGGGCATTCGCTT	GGAGTTGTAGTGCCCTTGGT
il-1β	XM_026220359.1	GAATGGAAACGACAGCCTCC	GGATTCGTTCAGTTGGCCTC
il-6α	XM_026252884.1	GAGATACCGACCACAGCTCA	TGCCCAACTGACTGCATAGA
il-8	KC184490.1	CACAAGTGTCGAGCAACCAG	TCAGTTTCAATGCAGCGACA
il-10	HQ259106	TGAAAAGGAACGATGGGCAG	TGGAATGATGACGTGCAAGC
tgf-β	EU086521.1	GGTTCTTGCGCTGTATAGGC	CCGGCCCACATAGTAAAGGA
zo-2	XM_026269460.1	ATGCGTCTGGGAATTACGGG	CATTCCTGAGCCCTTTCCCT
occludin-a	HQ110086.1	GGACCAGATCAACAAGCGTC	TTGATGTGGCTGAGTTTGGC
claudin-b	HQ656008.1	ACCGGACAGATGCAGTGTAA	ATGATGCCCAGGATCCCAAT
claudin-c	HQ656009.1	AGAGTACTGGACAGACGCAG	GATCATCACACGGGCTTTGG
claudin-d	HQ656010.1	ACTGCCACAAGATCTCCAGG	GTCCCGTTCTTCAATGCAGT
claudin-e	HQ656011.1	AGAAAGCAAGGCAAAGGTGG	TCCCTGACGATGGTGTTAGT
claudin-h	HQ656012.1	ACGGCACAAGTAATCTGGGA	CCAAGATGACGGCAATGACC
y^+lat2	XM_026266738.1	ATCATCACTGGCCTGGTCAA	CTGTGACAATGGGCATGGAG
snat2	XM_026209285.1	TCACGATCAACACCGAGTCA	ACAGCCCAAATGTGCGAAAT
pept1	XM_026265622.1	CCGTACTCATCCTCCCCATC	TCTCGGTCTCTCCTTCCTCA
casr	AB713518.1	AACTCCTGGTCTAACGGCAA	AACACCCAACACGAAAGCTG
gprc6a	XM_026283875.1	ACGCTGTGTGTTTCATGCAT	GCAAACGATCACATACGGCT
t1r3	XM_026275959.1	TTCTGAGCAGCTGGAGAACA	CTCCACTGGACAACGCAAAA
t1r1	XM_026268818.1	TGAATGGTCTGATGAGGGCA	GTAAACACATGCTGCCACCA
mglur4	EU147495.1	CCAGTATCAGCACGACCTCT	AATCGGCGTGTCATTGTAGC

β-actin, reference gene; tnf-α, tumor necrosis factor α; il-1β, interleukin 1β; il-6α, interleukin 6α; il-8, interleukin 8; il-10, interleukin 10; tgf-β, transforming growth factor β; zo-2, tight junction protein ZO 2; y⁺lat2, Y⁺L amino acid transporter 2-like; snat2, sodium-coupled neutral amino acid transporter 2; pept1, antigen peptide transporter 1-like; casr, extracellular calcium-sensing receptor-like; gprc6a, G protein-coupled receptor, class C, group 6, member A; t1r3, taste receptor type 1 member 3-like; t1r1, taste receptor type 1 member 1-like; mglur4, glutamate receptor, metabotropic 4.