Effects of the Replacement of Dietary Fishmeal by the Blend of Tenebrio molitor Meal, Chlorella Meal, Clostridium Autoethanogenum Protein, and Cottonseed Protein Concentrate on Growth, Protein Utilization, and Intestinal Health of Gibel Carp (Carassius gibelio, CAS Ⅴ)

Table 7

Apparent digestibility coefficients of substance (%) of gibel carp fed the experimental diets.

Diets	Control	A33	A67	A100	B33	B67	B100	C33	C67	C100
Dry matter	55.96 ± 0.70^bc	57.46 ± 0.83^ab	49.75 ± 0.88^e	49.40 ± 1.13^e	52.53 ± 0.83^d	55.10 ± 0.72^bcd	58.88 ± 0.75^a	58.80 ± 0.78^a	55.87 ± 0.58^bc	54.05 ± 1.06^cd
Gross energy	61.15 ± 0.78^ab	62.16 ± 1.13^a	53.46 ± 0.79^e	53.73 ± 1.05^e	56.95 ± 0.74^d	57.41 ± 0.84^cd	60.09 ± 0.83^abc	62.66 ± 1.02^a	59.06 ± 0.68^bcd	54.17 ± 0.81^e
Crude protein	78.01 ± 0.41^c	82.68 ± 0.42^a	76.06 ± 0.58^d	72.50 ± 0.46^e	79.35 ± 0.47^b	79.38 ± 0.53^b	82.25 ± 0.37^a	79.45 ± 0.32^b	78.40 ± 0.02^bc	79.19 ± 0.35^bc
Total essential amino acid	84.58 ± 0.32^d	86.70 ± 0.33^c	83.37 ± 0.24^e	82.93 ± 0.33^e	85.24 ± 0.40^d	86.79 ± 0.12^bc	87.60 ± 0.10^b	85.31 ± 0.07^d	86.37 ± 0.28^c	85.44 ± 0.55^d
Total non-essential amino acid	83.15 ± 0.43^d	85.13 ± 0.27^bc	82.29 ± 0.37^e	78.61 ± 0.19^f	82.88 ± 0.13^de	84.86 ± 0.16^bc	87.69 ± 0.11^a	83.22 ± 0.40^d	85.62 ± 0.23^b	84.63 ± 0.10^c
Essential amino acids
Lysine	84.20 ± 0.60^dc	86.74 ± 0.33^a	84.10 ± 0.40^dc	83.16 ± 0.46^d	85.55 ± 0.28^ab	85.58 ± 0.38^ab	86.70 ± 0.28^a	84.39 ± 0.37^bcd	85.12 ± 0.36^bc	83.96 ± 0.28^dc
Methionine	97.08 ± 0.19^b	97.79 ± 0.59^ab	97.07 ± 0.57^b	96.79 ± 0.04^b	97.57 ± 0.07^ab	97.75 ± 0.08^ab	98.24 ± 0.10^a	97.54 ± 0.57^ab	97.7 ± 0.06^ab	97.79 ± 0.08^ab
Threonine	75.19 ± 0.79^e	77.77 ± 0.34^ab	75.61 ± 0.47^de	71.68 ± 0.38^f	76.84 ± 0.28^bcd	77.44 ± 0.29^bc	78.92 ± 0.41^a	76.11 ± 0.52^cde	76.53 ± 0.35^bcde	76.62 ± 0.54^bcde
Arginine	76.74 ± 1.73^bc	76.11 ± 0.82^bcd	71.15 ± 0.77^f	72.69 ± 0.97^def	72.44 ± 0.95^ef	80.88 ± 0.50^a	78.84 ± 1.45^ab	76.26 ± 1.29^bc	79.05 ± 0.80^ab	75.10 ± 1.22^cde
Leucine	82.75 ± 0.42^e	85.81 ± 0.45^b	82.21 ± 0.31^ef	81.36 ± 0.35^f	83.93 ± 0.32^d	85.27 ± 0.32^bc	86.97 ± 0.37^a	84.38 ± 0.14^cd	85.26 ± 0.26^bc	84.52 ± 0.22^bc
Isoleucine	82.32 ± 0.02^d	86.94 ± 0.42^a	83.39 ± 0.54^d	82.48 ± 0.33^d	84.76 ± 0.27^bc	84.66 ± 0.65^bc	85.77 ± 0.05^b	85.24 ± 0.22^bc	84.46 ± 0.19^c	85.62 ± 0.23^b
Valine	79.69 ± 0.20^cd	83.02 ± 0.55^a	75.74 ± 0.71^e	75.27 ± 0.50^e	81.01 ± 0.86^bc	82.53 ± 0.49^ab	83.30 ± 0.39^a	78.87 ± 0.63^cd	82.02 ± 0.31^ab	82.31 ± 0.28^ab
Phenylalanine	82.31 ± 0.77^cde	84.66 ± 0.85^ab	77.41 ± 0.37^f	79.99 ± 0.94^ef	82.68 ± 1.77^cde	84.65 ± 0.37^ab	87.65 ± 0.35^a	81.17 ± 1.36^ed	84.34 ± 0.91^cd	83.51 ± 1.18^cd
Histidine	84.11 ± 0.90^b	86.78 ± 1.56^ab	87.24 ± 0.46^a	84.28 ± 1.12^b	86.90 ± 0.47^ab	87.72 ± 0.84^a	88.18 ± 0.82^a	89.20 ± 1.29^a	87.60 ± 0.52^a	88.24 ± 0.16^a
Non-essential amino acids
Asparagine	82.13 ± 0.64^b	85.21 ± 0.29^d	81.64 ± 0.41^b	78.28 ± 0.05^a	83.80 ± 0.19^c	84.37 ± 0.33^cd	87.38 ± 0.45^e	83.78 ± 0.47^c	84.33 ± 0.30^cd	85.03 ± 0.22^d
Serine	80.37 ± 0.56^c	83.49 ± 0.34^ab	82.6 ± 0.44^b	79.23 ± 0.38^b	83.07 ± 0.31^ab	82.68 ± 0.38^b	84.33 ± 0.31^a	83.22 ± 0.55^ab	82.58 ± 0.35^b	83.76 ± 0.40^ab
Glutamic acid	88.21 ± 0.79^bc	89.75 ± 0.29^b	87.26 ± 0.24^cd	85.87 ± 0.01^d	88.56 ± 0.44^bc	89.52 ± 0.42^b	91.45 ± 0.42^a	88.67 ± 0.68^bc	89.88 ± 0.64^b	89.33 ± 0.64^b
Glycine	73.59 ± 0.80^d	76.92 ± 0.64^c	76.23 ± 1.06^c	68.22 ± 0.43^e	75.18 ± 0.50^cd	79.08 ± 0.39^b	84.62 ± 0.45^a	76.64 ± 0.75^c	80.83 ± 0.48^b	79.02 ± 0.45^b
Alanine	80.33 ± 1.13^b	81.51 ± 0.39^b	82.08 ± 0.63^b	74.72 ± 0.87^c	80.54 ± 0.76^b	81.73 ± 1.03^b	85.84 ± 0.72^a	81.97 ± 1.75^b	82.56 ± 0.50^ab	82.93 ± 1.97^ab
Tyrosine	83.77 ± 0.59^c	86.64 ± 0.73^a	84.58 ± 0.38^bc	80.43 ± 0.27^d	83.85 ± 0.94^c	86.51 ± 0.74^a	86.83 ± 0.19^a	84.10 ± 0.25^bc	85.69 ± 0.64^ab	85.51 ± 0.29^abc
Proline	77.77 ± 0.44^d	80.80 ± 0.59^bc	74.25 ± 1.74^e	72.32 ± 0.68^e	79.05 ± 0.69^cd	82.35 ± 0.76^b	88.02 ± 0.62^a	79.00 ± 0.75^cd	82.26 ± 0.95^b	80.24 ± 0.35^bcd
Cysteine	85.11 ± 1.01^a	84.80 ± 0.45^ab	78.65 ± 0.19^e	73.15 ± 0.37^f	74.65 ± 0.56^f	81.62 ± 1.16^cd	82.73 ± 0.79^bc	74.79 ± 1.13^f	86.23 ± 0.47^a	79.86 ± 0.23^de

Values are means ± SE (n = 3), and values in the same row with different letters a, b, c, d, e, and f are significantly different (). Apparent digestibility coefficients of substance (%) = 100 × [1−(fecal substance/dietary substance) × (Y₂O₃ in diet/Y₂O₃ in feces)].