Research Article
Effects of Dietary Supplement of Basil Extract on Biochemical and Immunological Parameters and Growth Performance in Oncorhynchus mykiss
Table 2
The sequences of primers and probes.
| | Primer/probe names | Sequence of primers and probes (5′-3′) | Target genes | PCR efficiencies |
| | IL1b_For | CGAGTTCAAGGACAAGGA | IL-1β | 1.97 | | IL1b_Rev | GACTCCAACTCCAACACTA | — | — | | IL1b_Probe | FAM-TCTTCCACAGCACTCTCCAGC-BHQ1 | — | — | | ELF1_For | AGAGCTTCCAGGAATACC | ELF1 | 2.03 | | ELF1_Rev | CCTTGATGACACCAACAG | — | — | | ELF1_Probe | FAM-CAGTCTGCCTCATGTCACGC-BHQ1 | — | — |
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Primers and probes were designed in this study by Beacon Designer software (PREMIER Biosoft, USA).
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