Growth and Hepatopancreas Health of Juvenile Chinese Mitten Crab (Eriocheir sinensis) Fed Different Levels of Black Soldier Fly (Hermetia illucens) Larvae Meal for Fish Meal Replacement

Table 1

Ingredient formulation of the feed formula (g/kg dry basis) and diet proximate composition (% dry matter, n = 3).

Ingredients	Experimental diets
	FM	BSFLM10	BSFLM20	BSFLM30	BSFLM40	BSFLM50
Fish meal^1	350	315	280	245	210	175
Black soldier fly larvae meal^2	0	57	113	169	226	282
Soybean meal	130	130	130	130	130	130
Cotton meal	130	130	130	130	130	130
Fish oil	30	24	18	12	6	0
Soybean oil	30	24	18	12	6	0
Lecithin	5	5	5	5	5	5
Cholesterol	5	5	5	5	5	5
α-Starch	170	170	170	170	170	170
Cellulose	54	44	35	26	16	7
Choline chloride	5	5	5	5	5	5
Butylated hydroxytoluene	1	1	1	1	1	1
Vitamin premix^3	40	40	40	40	40	40
Mineral premix^4	20	20	20	20	20	20
Alginate-Na	30	30	30	30	30	30
Total	1,000	1,000	1,000	1,000	1,000	1,000
Proximate composition (%)
Moisture	9.08	9.60	10.26	9.80	9.92	10.29
Crude protein	38.02	37.76	38.25	37.94	37.52	38.25
Crude lipid	9.54	10.00	9.57	9.56	9.48	9.58
Crude ash	11.19	11.63	11.67	11.51	11.72	11.66

¹The crude protein concent of fish meal is 68.52% (dry matter). ²The crude protein content of black soldier fly larvae meal is 42.61% (dry matter). ³Vitamin mix (per 100 g premix) retinol acetate, 0.043 g; thiamin hydrochloride, 0.15 g; riboflavin, 0.0625 g; niacin, 0.3 g; Ca pantothenate, 0.3 g; pyridoxine hydrochloride, 0.225 g; ascorbic acid, 0.5 g; para-aminobenzoic acid, 0.1 g; folic acid, 0.025 g; biotin, 0.005 g; cholecalciferol, 0.0075 g; α-tocopherol acetate, 0.5 g; menadione, 0.05 g; inositol, 1 g. All ingredients were filled with α-cellulose to 100 g. ⁴Mineral premix (per 100 g premix): KH₂PO₄, 21.5 g; NaH₂PO₄, 10.0 g; Ca(H₂PO₄)₂, 26.5 g; CaCO₃, 10.5 g; KCl, 2.8 g; MgSO₄·7H₂O, 10.0 g; AlCl₃·6H₂O, 0.024 g; ZnSO₄⋅7H₂O, 0.476 g; MnSO₄⋅6H₂O, 0.143 g; KI, 0.023 g; CuCl₂⋅·2H₂O, 0.015 g; CoCl₂⋅·6H₂O, 0.14 g; calcium lactate, 16.50 g; Fe-citrate, 1 g. All ingredients were diluted with α-cellulose to 100 g.