Growth and Hepatopancreas Health of Juvenile Chinese Mitten Crab (Eriocheir sinensis) Fed Different Levels of Black Soldier Fly (Hermetia illucens) Larvae Meal for Fish Meal Replacement

Table 4

Fatty acid composition of the hepatopancreas (% total fatty acids, n = 4) in juvenile E. sinensis fed different diets.

Fat acids	Experimental diets						ANOVA	Regression analysis
	FM	BSFLM10	BSFLM20	BSFLM30	BSFLM40	BSFLM50		Linear		Quadratic
							P-value	Adj. R^2	P-Value	Adj. R^2	P-Value
C12 : 0	ND^g	2.30 ± 0.00^a	4.39 ± 0.08^b	4.67 ± 0.15^b	8.01 ± 0.35^c	9.33 ± 0.05^d	<0.001	0.938	<0.001	0.938	<0.001
C14 : 0	4.59 ± 0.21	5.00 ± 0.49	5.36 ± 0.12	5.16 ± 0.23	5.29 ± 0.24	4.99 ± 0.37	0.662	−0.031	0.531	0.055	0.233
C15 : 0	0.58 ± 0.02^b	0.53 ± 0.03^b	0.43 ± 0.02^a	0.44 ± 0.02^a	0.41 ± 0.04^a	0.39 ± 0.01^a	<0.001	0.606	<0.001	0.646	<0.001
C16 : 0	30.32 ± 0.56^c	29.02 ± 1.22^bc	27.60 ± 0.24^ab	27.84 ± 0.64^ab	26.63 ± 0.50^a	26.39 ± 0.80^a	0.010	0.515	<0.001	0.516	0.001
C16 : 1	10.65 ± 0.20	10.95 ± 0.34	11.22 ± 0.15	10.84 ± 0.49	10.27 ± 0.32	10.33 ± 0.05	0.374	0.048	0.185	0.123	0.136
C17 : 0	0.54 ± 0.05^b	0.47 ± 0.06^b	0.26 ± 0.01^a	0.30 ± 0.03^a	0.24 ± 0.01^a	0.30 ± 0.03^a	<0.001	0.455	<0.001	0.678	<0.001
C17 : 1	0.35 ± 0.01	0.34 ± 0.01	0.35 ± 0.02	0.31 ± 0.02	0.33 ± 0.01	0.32 ± 0.01	0.399	0.085	0.096	0.041	0.253
C18 : 0	3.50 ± 0.15^b	3.00 ± 0.14^a	2.62 ± 0.08^a	2.91 ± 0.26^a	2.76 ± 0.09^a	2.60 ± 0.20^a	0.012	0.299	0.003	0.351	0.004
C18 : 1n-9	26.65 ± 0.30^a	26.70 ± 0.19^a	26.92 ± 0.27^a	29.88 ± 0.69^b	30.13 ± 1.16^b	34.55 ± 0.06^c	<0.001	0.752	<0.001	0.860	<0.001
C18 : 2n-6	15.69 ± 0.40^c	15.85 ± 0.39^c	15.04 ± 0.32^bc	14.84 ± 0.35^abc	14.46 ± 0.15^ab	13.84 ± 0.15^a	0.005	0.550	<0.001	0.540	<0.001
C18 : 3n-3	0.57 ± 0.02^c	0.54 ± 0.05^c	0.38 ± 0.00^b	0.36 ± 0.02^b	0.29 ± 0.01^a	0.27 ± 0.01^a	<0.001	0.830	<0.001	0.846	<0.001
C20 : 0	1.20 ± 0.19	1.28 ± 0.23	1.32 ± 0.09	1.31 ± 0.06	1.29 ± 0.05	1.11 ± 0.12	0.923	−0.062	0.729	−0.029	0.474
C20 : 4n-6	0.69 ± 0.06^b	0.45 ± 0.01^a	0.38 ± 0.03^a	0.49 ± 0.10^a	0.41 ± 0.07^a	0.42 ± 0.04^a	0.026	0.194	0.023	0.291	0.015
C20 : 5n-3	3.57 ± 0.05^e	2.86 ± 0.07^de	2.37 ± 0.12^d	1.94 ± 0.21^c	1.65 ± 0.08^b	1.34 ± 0.04^a	<0.001	0.901	<0.001	0.925	<0.001
C22 : 5n-3	0.16 ± 0.01^b	0.13 ± 0.01^a	0.02 ± 0.02^a	ND^g	ND^g	ND^g	0.001	0.746	0.001	0.833	0.001
C22 : 6n-3	1.55 ± 0.11^d	1.00 ± 0.07^cd	0.86 ± 0.07^bc	0.78 ± 0.17^b	0.56 ± 0.05^b	0.43 ± 0.05^a	<0.001	0.732	<0.001	0.766	<0.001
∑SFA	35.74 ± 0.49^a	37.75 ± 0.26^b	39.13 ± 0.34^c	39.58 ± 0.47^c	41.89 ± 0.56^d	42.18 ± 0.06^d	<0.001	0.876	<0.001	0.878	<0.001
∑MUFA	33.95 ± 0.21^a	35.48 ± 0.61^ab	35.81 ± 0.47^b	36.03 ± 0.52^b	36.35 ± 0.79^b	38.32 ± 0.92^c	0.004	0.531	<0.001	0.508	<0.001
∑PUFA	16.99 ± 0.59^d	16.36 ± 0.50^cd	15.42 ± 0.32^bc	15.20 ± 0.46^abc	14.73 ± 0.14^ab	14.12 ± 0.14^a	0.001	0.689	<0.001	0.680	<0.001
∑HUFA	5.47 ± 0.30^d	3.91 ± 0.39^c	3.61 ± 0.23^c	3.20 ± 0.46^bc	2.59 ± 0.19^ab	2.19 ± 0.11^a	<0.001	0.727	<0.001	0.745	<0.001
∑n-3FA	5.35 ± 0.33^e	4.27 ± 0.30^d	3.62 ± 0.20^cd	3.08 ± 0.39^bc	2.48 ± 0.13^ab	2.05 ± 0.08^a	<0.001	0.841	<0.001	0.851	<0.001
∑n-6FA	16.82 ± 0.48^d	16.28 ± 0.55^cd	15.66 ± 0.33^bcd	15.38 ± 0.39^abc	14.85 ± 0.18^ab	14.28 ± 0.12^a	0.003	0.674	<0.001	0.655	<0.001
∑n-3FA/∑n-6FA	0.32 ± 0.01^e	0.27 ± 0.01^de	0.24 ± 0.01^d	0.18 ± 0.01^c	0.16 ± 0.00^b	0.14 ± 0.00^a	<0.001	0.931	<0.001	0.940	<0.001

The values are the mean ± standard error (n = 4). Adj. R², adjusted R²; Linear, linear trend; Quadratic, quadratic trend. Means in the same line with different superscripts are significantly different ().