Aquaculture Nutrition / 2024 / Article / Tab 5 / Research Article
Growth and Hepatopancreas Health of Juvenile Chinese Mitten Crab (Eriocheir sinensis ) Fed Different Levels of Black Soldier Fly (Hermetia illucens ) Larvae Meal for Fish Meal Replacement Table 5 Primer pair sequences and product sizes of the genes used for real-time PCR.
Genes Position Primer sequence Product size (bp) References β-Actin Forward TCGTGCGAGACATCAAGGAAA 178 Lin et al. [21 ] Reverse AGGAAGGAAGGCTGGAAGAGTG — S27 Forward CCCCCAAGAAGATCAAGCACA 179 Lin et al. [21 ] Reverse CAGATGGCAGCGACCACAGTA — LITAF Forward TAAAGGCAAGGGAGGCTTCG 97 GenBank: KC011816.1 Reverse GAATGGAGCTTGAGGTGGCA — Relish Forward TCAGGATTCGGTGGCAACTC 105 GenBank: GQ871279.1 Reverse ATCTGCACTTGGACCGATGG — ILF2 Forward GGGAACTTCGATGCCTGTCA 101 GenBank: GU002546 Reverse ATGACCACGATGTCCGCTAC — Toll Forward CTCCTTCACCTGCCCTAACTGCT 88 GenBank: KC011816.1 Reverse CTCCAGTTTGTATTGCTGTGCGAAA — MyD88 Forward GCCATCGCAGTCGCCAAGTT 148 GenBank: KC019316.1 Reverse GGCATCCTGTTCATCCAGTTCTGAC — p38MAPK Forward TGGGAGGTGCCCAAGAGATA 94 GenBank: KF582665.1 Reverse TGGTGTTTGTTTTGGCGTCC —
S27 , ubiquitin/ribosomal S27 fusion protein; LITAF , lipopolysaccharide-induced TNF factor; ILF2 , interleukin enhancer binding factor 2; MyD88 , myeloid differentiation factor 88; p38MAPK , p38 mitogen-activated protein kinase.
