Cholesterol Modifies Nutritional Values and Flavor Qualities in Female Swimming Crab (Portunus trituberculatus)

Figure 3

Effects of dietary cholesterol levels on amino acid metabolism of female swimming crab. (a and b) Total amino acid contents (g/100 g dry matter) in hepatopancreas and muscle; (c and e) relative expression of genes involved in TOR pathway in hepatopancreas and muscle of female swimming crab; (d and f) relative expression of genes of aspartate aminotransferase (ast), alanine aminotransferase 1 (alt1), and L-threonine 3-dehydrogenase (tdh) in hepatopancreas and muscle of female swimming crab; (g and h) heat map of multidimensional correlation between amino acid contents and amino acid metabolism-related gene expression levels in hepatopancreas and muscle. The existence of “” indicates a significant difference between the two comparisons. Data are reported as the mean and SEM of four replicates. Columns represent means with bars indicating standard error. Values within the same row with different superscripts are significantly different . EAA, essential amino acids; NEAA, nonessential amino acids; FLAA, flavor amino acids, including aspartic acid, glutamic acid, glycine, and alanine; TAA, total amino acids. s6, ribosomal protein S6; akt, protein kinases B; s6k1, ribosomal protein S6 kinase1; tor, target of rapamycin; 4e-bp1, eukaryotic initiation factor 4E-binding protein-1; eif4e1a, eukaryotic translation initiation factor 4E-1A; eif4e2, eukaryotic translation initiation factor 4E-2; eif4e3, eukaryotic translation initiation factor 4E-3.

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