Black Soldier Fly Oil in Different Lipid Diets Could Regulate Tissue Lipid Metabolism and Fatty Acid Composition of Juvenile Mirror Carp

Table 3

Primers used for qPCR analysis.

Genes	Forward (5–3)	Reverse (5–3)	Accession number	Size (bp)
FAS	TGCTGGATGCTTTGTTTGAG	ACTACACCACCAGCGATTCC	KY378913.1	85
PPAR-γ	CTTCGTGAACCTGGACTTG	ATCTGACCGTAGGAGATGAG	XM_019096045.1	123
PPAR-α	CATGTCCCACA ACGCTATC	TGTCTGAGGTAGGCTTCAT	FJ849065.1	156
CPT-1	CAGATGGAAAGTGTTGCTAATGAC	TGTGTAGAAGTTGCTGTTGACCA	JF728839	172
ß-actin	GCAGATGTGGATTAGCAAGCAG	TTGAGTCGGCGTGAAGTGG	M24113.1	100

Notes: FAS = Fatty acid synthase; PPAR-γ = Peroxisome proliferator-activated receptor-γ; PPAR-α = Peroxisome proliferator-activated receptor-α; CPT-1 = Carnitine palmitoyltransferase−1.