Research Article

HPTLC Fingerprint Profile and Identification of Antidiabetic and Antioxidant Leads from Bauhinia rufescens L

Table 4

1H- and 13C-NMR assignments of compound 2 (700 MHz, DMSO) in comparison with reported data for rutin (quercetin-3-orutinoside) (100 MHz, CD3OD) [27].

PositionδH compound (2)δH rutinδC compound (2)δC rutin

2158.08158.22
3134.70134.52
4178.21178.39
5162.88162.48
66.446.10 s99.7099.72
7166.0166.58
86.776.28 s94.8494.90
9159.06158.91
10105.99105.32
1′120.73122.77
2′7.64 s119.34117.37
3′144.00144.32
4′150.00150.23
5′6.90 d6.85 d115.67115.46
6′7.77 d7.63 d120.51122.47
1″5.22–5.274.96 d103.69103.63
2″5.22–5.274.96 d76.0074.64
3″5.22–5.274.96 d70.65
4″3.11–3.97 m3.20–3.90 m70.13
5″3.11–3.97 m3.20–3.90 m70.01
1‴4.694.50 d101.53101.92
2‴4.694.50 d71.7971.32
3‴4.694.50 d
4‴3.11–3.97 m3.20–3.90 m
5‴3.11–3.97 m3.20–3.90 m
6‴3.11–3.97 m3.20–3.90 m

δ = chemical shift in ppm, s = singlet, d = doublet, and m = multiplet.