HPTLC Fingerprint Profile and Identification of Antidiabetic and Antioxidant Leads from Bauhinia rufescens L

Table 5

1H- and 13C-NMR assignments of compound 1 (500 MHz, DMSO) in comparison with reported data for kaempferol-3-O-rutinoside (100 MHz, CD3OD) [27].

Position	δH compound (2)	δH kaempferol-3-O-rutinoside	δC compound (2)	δC kaempferol-3-O-rutinoside
2		—	158.08	155.98
3		—	134.70	134.58
4		—	178.21	177.23
5		—	162.88	162.04
6	6.44 d	6.22 d	99.70	98.88
7		—	166.0	163.31
8	6.77 d	6.33 d	94.84	93.98
9		—	159.06	156.82
10		—	105.99	104.79
1′		—	120.73	121.39
2′	8.01	8.19 d	130.96	130.76
3′	6.90 d	6.92 d	112.50	113.40
4′	—	—	160.41	160.92
5′	6.90 d	6.92	115.67	114.87
6′	7.77 d	8.19 d	131.00	131.03
1″	5.22–5.27	5.02 d	103.69	102.11
2″	5.22–5.27	5.02 d	76.00	74.83
3″	5.22–5.27	5.02 d	70.65	75.48
4″	3.11–3.97 m	3.15–3.90 m	70.13	69.23
5″	3.11–3.97 m	3.15–3.90 m	70.01	77.65
6″	3.11–3.97 m	3.15–3.90 m		67.08
1‴	4.69	4.45 d	101.53	100.10
2‴	4.69	4.45 d	71.79	70.89
3‴	4.69	4.45 d
4‴	3.11–3.97 m	3.15–3.90 m
5‴	3.11–3.97 m	3.15–3.90 m

δ = chemical shift in ppm, d = doublet, and m = multiplet.