Research Article
An Evaluation of the Antibacterial Properties of Tormentic Acid Congener and Extracts From Callistemon viminalis on Selected ESKAPE Pathogens and Effects on Biofilm Formation
Figure 7
The effect of C. viminalis EtOH:H2O extract, DCM:MeOH extract, tormentic acid congener, and ciprofloxacin on extracellular DNA produced by P. aeruginosa (a) and S. aureus (b). Cells were incubated in a medium containing 0.80 µg/ml ciprofloxacin as well as 12.5 µg/ml of the crude extracts and tormentic acid congener for 72 h. The amount of extracellular DNA present was determined by staining the biofilms with propidium iodide and spectrophotometrically measuring the amount of dye. These are results after a 24 well plate was washed with phosphate-buffered saline. ANOVA was carried out using GraphPad Prism6 and Dunnett’s multiple comparisons test with a summary of ∗∗∗∗P value < 0.0001. Ciprofloxacin, tormentic acid congener, and extracts reduced extracellular DNA production in P. aeruginosa and S. aureus biofilms.
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